ORBECO SP600, Instruction Manual, Page: 264 / 334

262 SP600 Spectrophotometer 01/2008
Correct filling of the vial:
1.2.3 Guidelines for photometric measurements
1. Vials, caps and stirring rods should be cleaned thoroughly after each analysis to
prevent influences. Even minor reagent residues can cause errors in the test result.
2. The outside of the vial must be clean and dry before starting the analysis. Clean the
outside of the vials with a towel. Fingerprints or other marks will be removed.
3. If there is no defined vial for the blank, the zeroing and the test must be carried out with
the same vial as there may be slight differences in optical performance between
vials.
4. The vials must be positioned in the sample chamber for zeroing and test with the mark
on the vial aligned with the mark on the instrument.
5. Always perform zeroing and test with closed photometer lid.
6. Bubbles on the inside wall of the vial leads to incorrect measurements. To prevent this,
remove the bubbles by swirling the vial before performing the test.
7. Avoid spillage of water in the sample chamber. If water should leak into the instrument
housing, it can destroy electronic components and cause corrosion.
8. Contamination of the lens in the sample chamber can result in errors. Check at regular
intervals and – if necessary – clean the light entry surfaces of the sample chamber using
a moist cloth or cotton buds.
9. Large temperature differences between the instrument and the environment can lead to
errors – e.g. due to the formation of condensation in the area of the lens or on the
vial.
10. To avoid errors caused by stray-light do not use the instrument in bright sunlight.