ORBECO SP600, Instruction Manual, Page: 254 / 334

252 SP600 Spectrophotometer 01/2008
1.1 Methods
TOC HR
with MERCK Spectroquant
®
Cell Test, No. 1.14879.0001
50 – 800 mg/l TOC
Sample preparation:
1. Pipette 1 ml of water sample into a suitable glass-
beaker.
2. Add 9 ml deionized water and mix.
3. Fill the beaker with the same size drops by holding the
bottle vertically and squeeze slowly:
2 drops reagent TOC-1K and mix.
4. pH value of the solution must below 2.5.
If necessary adjust the pH with sulphuric acid.
5. Stir for
10 minutes
at medium speed (magnetic stirrer,
stirring staff).
Digestion:
6. Pipette
3 ml pre-prepared sample
into one reaction
tube.
7. Add
1 level microspoon of reagent TOC-2K
.
8. Immediately close the vial with an aluminium cap
tightly.
9. Heat vials, standing on its head , at 120°C in the pre-
heated reactor for
120 minutes
.
10. Wait for 1 hour before proceeding.
Do not cool down with water!
Performing test procedure:
11. Place the supplied blank in the sample chamber making
sure that the marks are aligned.
12. Press ZERO key.
13. Remove the vial from the sample chamber.
14. Place the cooled down vial in the sample chamber ma-
king sure that the marks are aligned.
15. Press TEST key.
The result is shown in the display in mg/l TOC.
Ø 16 mm
prepare Zero
press ZERO
Zero accepted
prepare Test
press TEST
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