ABX Pentra 80 RAB108EA, User Manual

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Pentra 80
8–14
Pentra 80 - User Manual - RAB108EA
4. Limitations
Whilst every effort is taken by ABX Diagnostics to investigate and indicate all known
interferenceís, it is by no means possible to guarantee that all interferenceís have
been identified. At all times, results should be validated and communicated only
once all information relating to the patient has been assessed and taken into account.
4.1. Maintenance
In Chapter 7. Maintenance , specific maintenance procedures are listed. The maintenance
procedures identified are mandatory for proper use and operation of the ABX PENTRA 80 .
Failure to execute any of these recommended procedures may result in poor reliabil-
ity of the system.
4.2. Blood specimens
Verification of any abnormal test result (including flagged results or results outside of the
normal range) should be performed using reference methods or other standard laboratory pro-
cedures for conclusive verification of the results. The sections below list known limitations
of automated blood cell counters which use the principles of impedance and light absorbance
as principles of measurement.
4.3. Known interfering substances
WBC :
◆
White Blood Cells (Leukocytes) :WBC results that exceed the linearity limits of the system
will require dilution of the blood sample (Leukemia sample followed by a leukopenia). Re-
assaying the diluted sample will help to obtain the correct assay value.
◆ Unlysed Red Cells - In some rare instances, the erythrocytes in the blood sample may not
be completely lysed. These non-lysed red blood cells may be detected on the WBC histo-
gram with an L1 alarm or as an elevated baseline on the side (leading edge) of the lym-
phocytes population. Non-lysed erythrocytes will cause a falsely elevated WBC count.
◆ Multiple myeloma - The precipitation of proteins in multiple myeloma patients may give
high WBC counts.
◆ Leukemia - A very low WBC count may result from this disease because of possible in-
creased fragility of the leukocytes leading to destruction of some of these cells during
counting. These white cell fragments will also interfere with the white cell differential pa-
rameters.
◆ Chemotherapy - Cytotoxic and immunosuppressive drugs may increase the fragility of the
leukocytes which may cause low WBC counts.
◆ Cryoglobulins - Increased levels of cryoglobulin that may be associated with myeloma,
carcinoma, leukemia, macroglobulinemia, lymphoproliferative disorders, metastic tumors,
autoimmune disorders, infections, aneurism, pregnancy, thromboembolic phenomena, dia-
betes, etc, which can increase the WBC, RBC or PLT counts and the HGB concentration. The