ABX Pentra 80 RAB108EA, User Manual, Page: 213 / 306

Description & Technology
Measuring principles
Pentra 80 - User Manual - RAB108EA
6–19
NEUTROPHILS : The Neutrophils are larger in size than the lymphocytes. The neutrophils con-
tain granular material in their cytoplasm along with a segmented nucleus. Due to these
cellular features, more light will show through these cells as they pass through the flowcell.
This affect will place the neutrophils higher on the optical Y-axis and spread them along the
volume X-axis according to their maturity. Hyper-segmentation and increased granules will
place these cells even higher on the optical Y-axis.
EOSINOPHILS : The Eosinophils are somewhat like the neutrophils. They contain granular ma-
terial and segmented nuclei within the cytoplasm. The granular material is colorized with the
ABX EOSINOFIX before they are passed through the light path in the flowcell. Due to the col-
orization action of the reagent, the eosinophils will be place in the highest part of the optical
Y-axis. Hyper-segmentation and increased granules will spread this population across the
right top of the matrix.
Additional parameters : LIC (Large Immature Cells) and ALY (Atypical Lymphocytes) com-
plete the matrix spectrum of cellular placement.
Immature granulocytic cells are detected by their larger volumes and by the increased gran-
ules, which allow more light to pass through the cells and increase the intensity of scattered
light. Therefore, cells such as metamyelocytes will be found to the right of the neutrophils
and almost at the same level. Myelocytes and promyelocytes will be found on the far right of
the matrix in the saturation position. The metamyelocytes, myelocytes, and promyelocytes
will all be classed as (LIC) and there given results will be included in the neutrophil value.
The Blast cells will be generally located to the right of the monocyte population and, as such,
will increase the (LIC) count. Small blast cells will be found between the normal lymphocyte
and mococyte populations (ALY).
Platelet aggregates and debris from RBC cell fragments are found in the background noise
area, at the lower left corner of the matrix. Most of the cell population thresholds are fixed
and give the normal limits for the normal leukocyte morphologies. Changes in the morphology
of a specific population will be indicated on the matrix by a shift in the corresponding
population.
A Blast alarm is generated from increased counts within the (LIC) area; this is correlated with
the Blast detection on the Basophil histogram.
Large lymphocytes are usually detected in the (ALY) ( Atypical Lymphocytes ) zone, where re-
active lymphoid forms, stimulated lymphocytes, and plasmocytes are also found as well..