ABX Pentra 80 RAB108EA, User Manual

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Pentra 80
6–12
Pentra 80 - User Manual - RAB108EA
▼ dilutions
Tab. 6–1: RBC/PLT dilutions
3.2.2. HGB Measurement
During the analysis cycle, lysing reagent is released into the Dilution chamber. This reagent
breaks down the RBC cell membrane and releases the Hemoglobin within the cell. The hemo-
globin, released by the lysing reagent, combines with the Potassium cyanide from the lysing
reagent to form a chromogenous cyanmethemoglobin compound. This compound is then mea-
sured through the optical part of the first dilution chamber by way of spectrophotometry at
a wavelength of 550nm.
Tab. 6–2: HGB measurement
▼ Results
Hemoglobin results are given as such: Absorbance value obtained from the sample x coeffi-
cient of calibration.
Technical characteristics of the RED BLOOD CELL and PLATELET counts
Initial blood volume 10 µl Method Impedance
Vol. ABX DILUENT 2500 µl Aperture diameter 50 µm
Final dilution rate** 1/10000 Count vacuum 200 mb
Temperature of reaction 35°C Count period 2 X 6 seconds
**: Two successive dilutions are carried out : Primary Dilution for RBC and PLT:
Blood (µl) 10 µl
Vol. ABX DILUENT 1700 dilution 1/170
Secondary Dilution RBC and PLT (from the primary dilution)
Dilution (µl) 42,5 µl
Vol. ABX DILUENT 2500 dilution 1/58,8
Final dilution: 1/170 x 1/58,8 = 1/10000
Technical characteristics for the HGB MEASUREMENT
Blood volume 10 µl Method Photometry
Vol. ABX DILUENT 1700 µl Wavelength 550 nm
Vol. ABX Lyse 400 µl
complement ABX DILUENT 400 µl
Final dilution rate** 1/250
Temperature of reaction 35°C