ABX Pentra 80 RAB108EA, User Manual

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Pentra 80
6–18
Pentra 80 - User Manual - RAB108EA
▼ Results
From the Absorbance and Resistive measurement of the leukopcytes, a matrix is developed
with cell volumes on the X-axis and optical transmission on the Y-axis. Study of the matrix
image allows a clear differentiation of 4 of the 5 leukocyte populations. Due to the low per-
centage of Basophils in comparison to the rest of the leukocytes, they have a separate
measurement of their own instead of being present in the matrix.
Fig. 6–20 LMNE matrix results
LYMPHOCYTES : The Lymphocytes are a very small round shaped cell with condensed cyto-
plasm and large nucleus. These cells are normally positioned in the lower part of the optical
Y-axis as well as the lower part of the volume X-axis because of their small size. The far left
side of the lymphocyte zone (LL) should normally be empty. Any detection of cells in the (LL)
zone will indicate Small lymphocytes, Platelet aggregates, NRBC’s (
Nucleated Red Blood Cells ),
and improperly adjusted flowcell alignment. Background noise may also be detected in this
zone if the interference is great.
MONOCYTES : The Monocytes are a very large irregular shaped cell with large convoluted nu-
clei. The nucleus contains folds and sometimes vacuoles. The cytoplasm is also large with
non-granular intra-cellular material. These cells will not scatter or absorb a large amount of
light when passing through the flowcell. They will therefore be positioned in the lower part
of the optical Y-axis. Because the monocytes are a large cell, they will be placed to the right
of the volume X-axis.
LMNE
abs
o
rb
an
ce
volume
EOSINOPHILS
NEUTROPHILS
MONOCYTES
ATYPICAL
LYMPHOCYTES
(ALY)
LYMPHOCYTES
LARGE IMMATURE
CELLS (LIC)