ABX Pentra 80 RAB108EA, User Manual

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Speci fications
Limitations
Pentra 80 - User Manual - RAB108EA
8–15
specimen must be warmed up to 37°C (99°F) in a bain marie for 30 minutes and analyzed
again immediately after (analyzer or manual method).
◆ Macrothrombocytes - in excessive numbers may affect and increase Leukocyte numera-
tion.
RBC :
◆ Red Blood Cells (Erythrocytes) : The red blood cell dilution contains all the formed ele-
ments in the blood: erythrocytes, leukocytes and platelets. During erythrocytes counting
(red blood cells), platelets are not counted as their size falls below the minimum threshold.
◆ Agglutinated erythrocytes - May cause a low incorrect RBC count. Blood samples contain-
ing the agglutinated red blood cells may be suspected by elevated MCH and MCHC values
and shown by examination of the stained blood film.
◆ Cold agglutinins - IgM immunoglobulins which are high in cold agglutinin disease may
cause lower RBC and PLT counts and increase MCV.
HGB (Hemoglobin) :
◆ Turbidity of the blood sample - Any number of physiological and/or therapeutic factors
may produce high incorrect HGB results. To obtain accurate hemoglobin results when in-
creased turbidity of the blood sample occurs, determine the cause of the turbidity and fol-
low the appropriate method below:
◆ High WBC : An extremely high WBC will cause excessive light scatter. In these cases use
reference (manual) methods.The diluted sample should be centrifuged, and the superna-
tant fluid measured with a spectrophotometer.
◆
High lipid concentration : A high concentration of lipids in the blood sample will give the
plasma a «milky» appearance. This condition can occur with hyperlipidemia, hyperpro-
teinemia (as in gammapathies) and hyperbilirubinemia. Accurate hemoglobin determina-
tions can be achieved by using reference (manual) methods and a plasma blank.
◆ Increased turbidity may also be seen in cases where the red blood cells are resistant to
lysing. This condition will cause an incorrect high HGB result, but may be detected by ob-
serving the abnormal MCH, MCHC values, and the increased baseline on the leading edge
of the WBC histogram. Erroneous hemoglobin results will cause the results of the MCH and
MCHC to be incorrect as well.
◆ Fetal bloods - The mixing of fetal and maternal bloods may produce a high inaccurate HGB
value.
HCT (Hematocrit) :
◆ Red blood cells agglutination - May produce an inaccurate HCT and MCV values. Red blood
cell agglutination may be detected by observing abnormal MCH and MCHC values, as well
as by examination of the stained blood film In such cases, manual methods may be required
to obtain an accurate HCT value