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KMS-310/400 Supervisor's Manual
6-47
Column Focus Command
The column focus function is useful in finding an image with low magnification
objectives over an extended range, ordinarily more than 100 microns. Though column
moves are motor-encoder based, the function returns a Z-scale position value. The
entire video field is metered for peak intensity.
!
Caution: The column focus function can crash the optical objective into the specimen.
The positive (lower) hardware Z-limit switch MUST be set before this function can be
safely used. To set the Z-positive limit, proceed as follows:
1.
Manually focus upon the highest feature of the specimen, using the
highest (installed) magnification objective.
2.
Note the Z-scale reading (in tenth microns).
3.
Drive the objective 100 microns closer (up arrow key with FOCUS ICON
selected) to the specimen.
4.
Using an 1/8-inch Allen key, set the Z-limit so that the limit LED
illuminated at this point.
Table 6-25. Column Focus Command
Script Name
Functional Category
Page Number
FOCUSIMG Vsets
Vtype
Seek peak focus using column (minimum step is
100 nm). If Vsets, uses FF_SET values; if Vtype,
uses edge contract; otherwise uses peak intensity
(confocal).
6-57
Summary of Contents for KMS-310
Page 10: ...Contents viii...
Page 33: ...System Overview Product Overview Subsystem Overview Functional Overview 3...
Page 51: ...User Interface Overview Software Controls Software Organization 4...
Page 119: ...Creating Automated Scripts Overview Script Creation Script Locator Script Commands 6...
Page 266: ...7 48 Chapter 7 Operation...
Page 292: ...8 26 Chapter 8 Maintenance...
Page 293: ...Error Messages System Error Messages Script Error Messages 9...
Page 297: ...Glossary...
Page 304: ...I 4 Index...