Chapter 4, Safety and Patient Care Precautions
69
Haemonetics
®
Cell Saver
®
Elite
®
User Manual
P/N 120745-IE, Manual revision: AA
Note: In the event of a bowl overfill, the device tries to reduce the amount of
RBC spillage, which may result in an extended Wash phase and longer
procedure time.
To avoid overfilling the bowl when Auto-Wash is turned off:
1. Carefully watch the RBC layer as the bowl fills.
2. Touch
Wash
to manually start Wash when the RBC layer is close
1
to the
bowl optics beam.
Note: The hematocrit of the product may be reduced if the Wash phase is
started before the bowl is full.
Caution:
A Wash flow rate that is too low provides a poor wash of the cells due
to insufficient agitation and mixing of saline solution with the RBC layer.
2.)
Excessive flow rate of saline solution due to processing parameters set by
the user.
Note: Haemonetics recommends the Wash speed be at least 25 mL/min lower
than the Fill speed for the 125 mL and 225 mL bowls. This ensures the cells
are packed more forcefully during Wash and therefore less likely to spill.
3.)
Pump regulation is disabled.
4.)
The pump has paused, the RBC layer is close
1
to the bowl optics beam, and
the user restarts the Fill phase or enters the Concentrate phase.
If the pump has paused and the RBC layer is close
1
to the bowl optics beam
and the user restarts the Fill phase or enters the Concentrate phase, the RBCs
may start to spill into the waste bag and the device will not transition into the
Wash phase when expected.
When entering the Concentrate phase the centrifuge speed slows down,
causing the RBC layer to expand. If the RBC layer is close
1
to the bowl optics
beam, this expansion may push the RBC layer past the bowl optics beam.
When entering the Concentrate phase, and when restarting the Fill phase,
there is a blind volume
2
when the bowl optics and line sensor are not active. If
the RBC layer passes the bowl optics trip point during this blind volume, the
device does not enter the Wash phase until the line sensor detects RBCs. By
1.
Within:
• 6 mm (125 mL or 225 mL bowl)
• 3 mm (70 mL bowl)
2.
A blind volume is a period of time when the sensor (either the bowl optics sensor or
the line sensor) does not detect fluid flowing past it; the sensor does not trigger any
actions during the blind volume. The purpose of the blind volume is to prevent a
premature transition to the Wash phase while the RBC layer stabilizes.
The blind volume is:
•
125/225 mL bowl:
25 mL (bowl optics), 25 mL (line sensor)
•
70 mL bowl:
35 mL (bowl optics), 35 mL (line sensor)
