Chapter 6, General Operation: Sequestration
111
Haemonetics
®
Cell Saver
®
Elite
®
User Manual
P/N 120745-IE, Manual revision: AA
collection bag harness at the appropriate times. What clamps to open and
close depend on whether you have chosen to collect platelet poor plasma
or return it to the RBC bag in the protocol settings.
Note: The white, blue, and yellow clamps should never be closed at the same
time.
Note: The prompts below correspond to the Haemonetics default settings and
may appear differently if using other parameters.
3. Check that the red ratchet clamps on the collection bags are closed.
4. Following the prompts on the screen,
a. Open the white air line clamp and close the yellow PPP line and blue
PRP line clamps when prompted.
b. Agitate the blood bag.
c. Touch
Continue
to start the Fill phase. The pump starts drawing blood.
As the bowl fills, plasma becomes visible as the first layer followed by
a white band (platelets), then RBCs.
Figure 56, Example of a Sequestration process prompt
5. Observe the centrifuge bowl filling and allow air from the processing set
to flow to the air bag or PPP collection bag.
Collecting PPP
The device displays another set of prompts when plasma reaches the effluent
line sensor.
