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Setting Up the Eluent Generator

Doc. 065294-01 3/09

Installing the EluGen Cartridge and Preparing for Conditioning

Follow the steps in this section to install the EluGen cartridge and prepare
it for conditioning before initial use.

Figure 83

illustrates the liquid flow path during the conditioning

procedure.

Figure 83. EluGen Cartridge Plumbing for Conditioning with Carbonate Eluent

EGC

DEGAS

EGC

Connect to

Gas Separator

Assembly

TO DEGAS

ELUENT OUT

TO SRS/AES

REGEN OUT

WASTE,

GAS

SEPARATOR

Connect to

Suppressor

Regen Out

Pre-plumbed

Pump Pulse

Damper

Inlet

Outlet

EGC VENT

Send to waste

EGC
OUT

EGC II

K CO

TO DEGAS
ELUENT IN

Back-

pressure

Coil

TO INJ

VALVE IN - P/

ELUENT OUT

To waste

14 MPa (2000 psi)
at 1.0 mL/min

TO PUMP/

DAMPER

Summary of Contents for ICS-2100

Page 1: ...ICS 2100 Ion Chromatography System Installation Instructions Document No 065294 Revision 01 March 2009...

Page 2: ...PUBLICATION IS PROVIDED AS IS WITHOUT WARRANTY OF ANY KIND DIONEX CORPORATION DOES NOT WARRANT GUARANTEE OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE OR THE RESULTS OF THE USE OF...

Page 3: ...romeleon Server 12 9 Connecting to the Chromeleon PC 13 9 1 Connecting the IC System to the PC 13 9 2 Connecting Additional USB Devices 14 10 Connecting the Power Cord 16 11 Installing the ICS 2100 De...

Page 4: ...nt Generator 38 18 1 Eluent Generator Setup for KOH LiOH MSA and NaOH Eluents 38 18 2 Eluent Generator Setup for Carbonate Bicarbonate Eluent 54 18 3 Eluent Generator Setup for Carbonate Eluent 80 19...

Page 5: ...C 40 to 104 F Humidity 5 to 95 relative humidity noncondensing Provide a sturdy workbench for the system of a height that ensures convenient access to the interior of the ICS 2100 Allow at least 15 cm...

Page 6: ...acking the IC System 1 Open the shipping box see Figure 1 and remove the top layer of foam Figure 1 Unpacking the ICS 2100 2 Open the plastic bag that covers the IC system see Figure 2 Figure 2 IC Sys...

Page 7: ...ting the IC system Figure 4 IC System with Ship Kit 7 Lift the IC system off the bottom layer of foam and out of the box Set it on a workbench Two or more persons should lift the IC system which weigh...

Page 8: ...n the appropriate section below configure the valve as the injection valve in Chromeleon For instructions refer to Installing the ICS 1100 1600 2100 Auxiliary Valve Document No 065288 The document is...

Page 9: ...valve in the IC system This causes unnecessary wear on the autosampler valve If your AS has an injection valve contact Dionex for assistance 1 Place the autosampler to the left of the IC system on the...

Page 10: ...pler inject port to the IC system injection valve is specially constructed It consists of two tubes one inside the other that are sealed at the end This seal must be maintained In addition the line is...

Page 11: ...from the front 7 Bend the green waste line about 5 mm 0 2 in from the end to ensure it stays in the AS drip tray When bending the tubing be sure not to restrict the inner diameter 8 Push the end of th...

Page 12: ...tions go to http www dionex com select Software under Products and click the Specifications link 2 Verify that the Chromeleon disk contains Chromeleon version 6 80 SR6a or later 3 For a local PC Log o...

Page 13: ...to install Chromeleon a On the Chromeleon Setup screen click Exit b Go to the following folder on the Chromeleon disk CM680SRx where x is the service release number For example if you are installing...

Page 14: ...is issued with a USB dongle plug the dongle into a USB port on the PC Always connect the USB dongle to the PC not to a Dionex module Microsoft Windows automatically detects the USB dongle and launche...

Page 15: ...the license key code an alphanumeric series provided on the Software License key code sheet Store the key code sheet in a safe place 1 Click Start on the Windows taskbar and select All Programs Chrom...

Page 16: ...d the server is not running Right click the icon and select Start Server If the icon is gray the server is already running If the Server Monitor icon is not on the Windows taskbar follow these steps t...

Page 17: ...em Ship Kit 2 Plug the cable s A connector into a USB port on the Chromeleon PC and plug the cable s B connector into the USB receptacle labeled FROM PC on the IC system rear panel see Figure 13 Do no...

Page 18: ...anel are provided for connecting additional Dionex USB compliant modules for example an autosampler to the system To connect an additional USB module plug the A connector on a USB cable into an A port...

Page 19: ...ations Use a hub if the number of USB devices in the system exceeds the number of available USB ports Use a hub if the IC system or other USB device is more than 5 meters 16 ft from the PC Figure 15 E...

Page 20: ...e Make sure the socket outlet is located near the IC system and is easily accessible Operation at AC input levels outside of the specified operating voltage range may damage the IC system DANGER D LEC...

Page 21: ...el see Figure 14 to turn on the power 5 Turn on the power to the autosampler if installed 6 Windows automatically detects the new USB devices A message flashes on the screen to inform you that new har...

Page 22: ...al devices were found for example an autosampler complete the wizard for the device 12 Setting Up Chromeleon NOTE This section provides brief instructions for setting up Chromeleon For details about a...

Page 23: ...e new timebase appears under the server name 6 Right click the new timebase and select Add Device The Add device to timebase dialog box appears 7 Under Manufacturers select Dionex IC Integrated System...

Page 24: ...Instructions 20 Doc 065294 01 3 09 9 Click the arrow next to Module Serial No In the list select the serial number of the system you are configuring see Figure 19 Figure 19 Example Properties Dialog...

Page 25: ...onfigure an auxiliary power supply optional To operate an electrolytic device such as a water polisher you can configure the EGC 2 power supply as an auxiliary power supply The power supply operates i...

Page 26: ...figured information about the power supply current on off etc is displayed in Chromeleon and on the front panel touch screens 2 Click the Inject Valve tab and verify that the correct device is assigne...

Page 27: ...ect the device 3 After selecting the device properties click OK to close the dialog box The IC system is added to the timebase see Figure 23 4 If you are configuring an autosampler add the autosampler...

Page 28: ...sing manual injections Close the Server Configuration Check dialog box 7 Exit the Server Configuration program 12 3 Connecting to the Panel Tabset 1 Click Start on the Windows taskbar and select All P...

Page 29: ...rate Control panel for the autosampler is included in the panel tabset 12 4 Verifying Chromeleon Communication Verify that Chromeleon is communicating with the IC system by clicking the Inject and the...

Page 30: ...structions refer to the column and suppressor manuals The manuals are provided on the Dionex Reference Library CD ROM P N 053891 For reference Figure 26 shows the system flow schematic after all plumb...

Page 31: ...any tubing temporarily connecting the suppressor ports 13 2 Setting Up the Column Heater Note for 2 mm columns For best results with 2 mm columns install a microbore heat exchanger P N 060943 in the c...

Page 32: ...Cut off the two shipping ties from the column heater and remove them see Figure 27 2 Loosen the two thumbscrews on the heater cover they remain attached to the cover 3 Pull the heater cover straight o...

Page 33: ...Check the arrow on the guard column label it should point down flow away from the injection valve 3 Connect the GUARD OUT line to the guard column outlet 4 Push the guard column onto the back column...

Page 34: ...e slightly out of the opening Connect this cable to the cable from the suppressor align the pins on the two connectors and push them together Figure 29 Suppressor Mounting Tabs and Guidelines 3 Orient...

Page 35: ...essor 9 Do not connect the REGEN OUT port until after completing the eluent generator setup Section 18 10 Replace the cover on the column heater making sure the GUARD IN line is aligned with the slot...

Page 36: ...ess that results in the production of small amounts of oxygen or hydrogen gas To ensure that the gas is not trapped in a closed container and allowed to concentrate install a 1 3 cm 0 52 in ID black w...

Page 37: ...ver if eluent is manually degassed or is sensitive to contamination Dionex recommends pressurizing the reservoir with helium or nitrogen See Section 22 for instructions VERSCHLIESSEN SIE DEN ABFALLBEH...

Page 38: ...and updates the Eluent Bottle volume as the eluent is used up A warning appears if the level falls below 200 mL Warnings are repeated at 100 mL and 0 mL If preferred you can enter the eluent bottle l...

Page 39: ...tallation after changing eluents or when eluent lines are empty Priming the pump heads with the Prime button see Section 17 2 Perform this procedure after the eluent lines are primed 17 1 Priming the...

Page 40: ...re 34 5 Under Eluent Flow Valve click Open 6 Draw the syringe back to begin pulling eluent through the flow path It may take several syringe draws to remove all air or previous eluent from the tubing...

Page 41: ...e Prime button Confirm that the waste valve is open by clicking OK when the reminder message appears The pump will begin pumping at approximately 3 mL min 4 Continue priming the IC system until no air...

Page 42: ...ns in this section if you are installing a KOH LiOH MSA or NaOH EluGen cartridge and a CR TC If you are installing a K2CO3 cartridge an EPM and an EGC CO3 Mixer for generating carbonate bicarbonate el...

Page 43: ...TC you will need the following items to complete the eluent generator setup Item P N Use To Yellow 0 5 mL min 7 MPa 1000 psi backpressure coil supplied in the ICS 2100 Ship Kit P N 064375 053765 Prov...

Page 44: ...40 Doc 065294 01 3 09 Figure 36 illustrates the ICS 2100 after the EluGen cartridge is installed Figure 36 ICS 2100 with an EluGen Cartridge Installed Top View IN EGC OUT EluGen Cartridge EGC EGC Hold...

Page 45: ...IN Connect to Gas Separator Assembly TO DEGAS ELUENT OUT TO CR TC REGEN OUT blue TO SRS AES REGEN OUT TO CR TC REGEN IN orange WASTE GAS SEPARATOR DEGAS ELUENT OUT TO INJ VALVE IN P ELUENT OUT Connec...

Page 46: ...metal ball stud inside the bottom of the cartridge holder see Figure 38 and the bracket on the CR TC mounting plate see Figure 39 These are used to mount the CR TC inside the holder after it is plumbe...

Page 47: ...ings and ferrules on the lines remove the coupler from each line just before connecting the line to a port Figure 40 Plumbed CR TC 5 Check that none of the lines are caught under the CR TC and then pu...

Page 48: ...Turn the EGC holder right side up and set it back into position Installing the EluGen Cartridge 1 Orient the EluGen cartridge with the fittings facing up and remove the plugs from the EluGen cartridge...

Page 49: ...the EGC holder see Figure 44 3 Pull the coiled black EGC IN and EGC OUT lines out slightly from the cartridge holder 4 Disconnect the EGC IN line from the union that connects it to the EGC OUT line 5...

Page 50: ...ge vigorously and tap it with the palm of your hand 10 to 15 times Check that all bubbles trapped in the eluent generation chamber are dislodged The EluGen cartridge contains one of the following a co...

Page 51: ...he cartridge when you slide it into the holder The lines are designed to coil inside the holder Figure 47 Installing the EluGen Cartridge in the Holder 10 Remove the plug from the EGC 1 connector on t...

Page 52: ...pening Remove the cap on the luer adapter by twisting Save the cap 4 Twist the luer lock and EluGen cartridge vent line into the luer adapter see Figure 50 Figure 50 Installing the EluGen Cartridge Ve...

Page 53: ...e Chromeleon Server Configuration program select Start Programs Chromeleon Server Configuration 2 Double click the ICS 2100 system icon The properties dialog box for the ICS 2100 appears 3 Click the E...

Page 54: ...he ICS 2100 touch screen SUPPRESSOR page and ELUENT GENERATOR page Disabling these options lets you turn on the pump without automatically turning on the suppressor and CR TC 6 Verify that the eluent...

Page 55: ...e EluGen cartridge Check for bubbles if necessary shake and tap the EluGen cartridge to remove bubbles 18 Reinstall the EluGen cartridge in the holder 19 Do not reconnect the EluGen cartridge cable un...

Page 56: ...the Automatically turn on with pump option is not selected on the ICS 2100 touch screen SUPPRESSOR page Disabling Figure 53 CR TC Plumbing for Hydration To avoid damaging the suppressor always turn of...

Page 57: ...t P 2 on the injection valve 3 Reconnect the EluGen cartridge cable 4 Remove the plug from the CR TC connector on top of the ICS 2100 5 Orient the CR TC s black 3 pin electrical cable connector as sho...

Page 58: ...MSA or NaOH EluGen cartridge and a CR TC go to Section 18 1 Overview The eluent generator setup procedure for carbonate bicarbonate eluent consists of the following main steps Installing the EluGen ca...

Page 59: ...EPM REGEN OUT port EluGen Cartridge Vent Line Tubing Kit supplied with the cartridge 063092 Vent the cartridge during operation Yellow 0 5 mL min 7 MPa 1000 psi backpressure coil 053765 Provide backpr...

Page 60: ...tallation Instructions 56 Doc 065294 01 3 09 Figure 55 illustrates the EluGen cartridge and EPM after installation Figure 55 ICS 2100 with an EluGen Cartridge and EPM Installed EluGen Cartridge EGC EG...

Page 61: ...VENT TO DEGAS ELUENT IN EGC DEGAS Connect to Gas Separator Assembly Connect Inject Valve Port P 2 to Connect to Suppressor Regen Out Pre plumbed Pump Pulse Damper to Inlet Outlet Send to waste EGC CO...

Page 62: ...ns 58 Doc 065294 01 3 09 Installing the EluGen Cartridge 1 Tilt the cartridge holder to view the bottom and brace the holder against the back corner of the ICS 2100 see Figure 57 Figure 57 Cartridge H...

Page 63: ...EK tubing red labels P N 064334 to the coupler see Figure 58 b Connect the 35 cm 14 in length of black PEEK tubing yellow labels P N 064340 to the coupler on the TO CR TC ELUENT OUT line yellow label...

Page 64: ...de of the holder see Figure 59 5 Turn the EGC holder right side up and set it back into position 6 Orient the EluGen cartridge with the fittings facing up and remove the plugs from the cartridge INLET...

Page 65: ...the EGC IN line from the coupler that connects it to the EGC OUT line 9 Connect the EGC IN line to the EluGen cartridge INLET port see Figure 62 10 Remove the coupler and connect the EGC OUT line to t...

Page 66: ...nstalled in the Holder The EluGen cartridge contains one of the following a corrosive base KOH LiOH or NaOH a corrosive acid MSA or a concentrated K2CO3 solution Wear protective eyewear and gloves whe...

Page 67: ...the cable connector fingertight to secure it Figure 64 Connecting the EluGen Cartridge Cable Connecting the EluGen Cartridge Vent Line 1 Locate the EluGen Cartridge Vent Line Tubing Kit supplied with...

Page 68: ...To prevent leaks from the EluGen cartridge always replace the luer adapter cap before moving the EluGen cartridge 6 Direct the vent line to the waste container and insert it down into the waste gas s...

Page 69: ...lled on EGC Holder 4 Connect the following lines to the EPM Connect the TO CR TC EPM ELUENT OUT line yellow label to the ELUENT OUT port on the EPM see Figure 69 Connect the TO CR TC EPM ELUENT IN lin...

Page 70: ...as Separator Assembly Pre plumbed Pump Pulse Damper to Inlet Outlet Send to waste EGC II K CO 2 3 TO CR TC EPM REGEN IN orange label TO PUMP DAMPER TO CR TC EPM ELUENT OUT yellow label TO CR TC EPM RE...

Page 71: ...on program select Start Programs Chromeleon Server Configuration 2 Double click the ICS 2100 system icon The properties dialog box for the ICS 2100 appears 3 Click the Eluent Generator tab see Figure...

Page 72: ...DEGAS ELUENT OUT WASTE GAS SEPARATOR TO INJ VALVE IN P ELUENT OUT EGC VENT TO DEGAS ELUENT IN EGC DEGAS Connect to Gas Separator Assembly Pre plumbed Pump Pulse Damper to Inlet Outlet Send to waste E...

Page 73: ...sure coil 4 Disconnect the TO SRS AES REGEN OUT line from the suppressor REGEN OUT port 5 Connect the TO SRS AES REGEN OUT line to the 10 32 to 1 4 28 coupler see Figure 72 NOTE The backpressure coil...

Page 74: ...e pump flow rate to 1 00 mL min 5 Turn on the pump 6 Run the pump for 5 minutes to hydrate the EPM 7 Touch the EGC button on the HOME page to open the ELUENT GENERATOR page 8 Verify that the cartridge...

Page 75: ...urn off the EGC 1 and EGC 2 current 13 Turn off the pump Installing the EGC CO3 Mixer Figure 73 illustrates the EGC CO3 Mixer after installation Figure 73 ICS 2100 with an EGC CO3 Mixer Installed TO E...

Page 76: ...the outlet pointing upward and push it onto the clips see Figure 75 The mixer outlet must point upward to ensure thorough mixing of the eluent 4 Locate the 35 cm 14 in length of black PEEK tubing whit...

Page 77: ...erify that the EGC 1 and EGC 2 currents are off 2 Disconnect the EluGen cartridge inlet line labeled TO PUMP DAMPER from the coupler that connects it to the green pump outlet line see Figure 76 Figure...

Page 78: ...n the pump without automatically turning on the suppressor 7 Set the pump flow rate to 5 00 mL min 8 Turn on the pump 9 Run the pump until the mixer is filled and there is a consistent flow of water e...

Page 79: ...luGen Cartridge and EPM Plumbing for Initial Filling of the EGC CO3 Mixer with Carbonate Bicarbonate Eluent EGC IN TO DEGAS ELUENT OUT WASTE GAS SEPARATOR TO INJ VALVE IN P ELUENT OUT EGC VENT TO DEGA...

Page 80: ...ckpressure coil from the TO SRS AES REGEN OUT line and then connect the backpressure coil to the mixer inlet see Figure 79 5 Connect the mixer outlet to the TO SRS AES REGEN OUT line NOTE The backpres...

Page 81: ...n to 3 5 mM and the EGC 2 concentration to 1 0 mM 12 Turn on the EGC 1 and EGC 2 currents 13 Run at the selected settings until the mixer is filled with the eluent mixture At a flow rate of 1 mL min i...

Page 82: ...low Schematic Carbonate Bicarbonate Eluent Generation Eluent In Regen In Regen Out Eluent Out L L S W P C EPM Eluent In Eluent Out Regen Out Regen In To the pump Degas In Cell In Cell Out Column Heate...

Page 83: ...the back of the component panel see Figure 81 3 Connect the mixer outlet line to port P 2 on the injection valve NOTE Backpressure tubing may be required between the injection valve and the EGC CO3 Mi...

Page 84: ...R TC go to Section 18 1 If you are installing a K2CO3 cartridge an EPM and an EGC CO3 Mixer for generating carbonate bicarbonate eluent go to Section 18 2 Overview The eluent generator setup procedure...

Page 85: ...g operation EGC CO3 Mixer mounting clips 063642 Install the mixer Phillips head screwdriver not supplied n a Install the EGC CO3 Mixer mounting clips 35 cm 14 in of black 0 25 mm 0 010 in ID PEEK tubi...

Page 86: ...GAS EGC IN Connect to Gas Separator Assembly TO SRS AES REGEN OUT WASTE GAS SEPARATOR Pre plumbed Pump Pulse Damper to Inlet Outlet EGC VENT Send to waste EGC OUT EGC II K CO 2 3 TO DEGAS ELUENT IN Ba...

Page 87: ...path during the conditioning procedure Figure 83 EluGen Cartridge Plumbing for Conditioning with Carbonate Eluent EGC DEGAS EGC IN Connect to Gas Separator Assembly TO DEGAS ELUENT OUT TO SRS AES REGE...

Page 88: ...remove the plugs from the cartridge INLET and OUTLET fittings see Figure 84 Figure 84 EluGen Cartridge Fitting Plugs 2 Set the EluGen cartridge in the service area on top of the ICS 2100 and orient i...

Page 89: ...palm of your hand 10 to 15 times Check that all bubbles trapped in the eluent generation chamber are dislodged The EluGen cartridge contains one of the following a corrosive base KOH LiOH or NaOH a c...

Page 90: ...hen you slide it into the holder The lines are designed to coil inside the holder Figure 87 EluGen Cartridge Installed in the Holder 8 Remove the plug from the EGC 1 connector on top of the ICS 2100 9...

Page 91: ...53765 see Figure 89 12 Direct the free end of the backpressure coil to a temporary waste container Installing the EluGen Cartridge Vent Line 1 Locate the EluGen Cartridge Vent Line Tubing Kit P N 0630...

Page 92: ...cartridge vent line into the luer adapter see Figure 91 Figure 91 Installing the EluGen Cartridge Vent Line NOTE To prevent leaks from the EluGen cartridge always replace the luer adapter cap before...

Page 93: ...Configuration program click Start on the Windows taskbar and select All Programs Chromeleon Server Configuration 2 Double click the ICS 2100 system icon The properties dialog box for the ICS 2100 app...

Page 94: ...S 2100 touch screen HOME page set the pump flow rate to 1 00 mL min 5 Turn on the pump 6 On the touch screen HOME page touch the EGC button to open the ELUENT GENERATOR page Verify that the cartridge...

Page 95: ...tor Doc 065294 01 3 09 91 Installing the EGC CO3 Mixer Figure 93 illustrates the EGC CO3 Mixer after installation Figure 93 ICS 2100 with an EGC CO3 Mixer Installed TO EGC CO3 MIXER IN P N 064346 TO E...

Page 96: ...ith the outlet pointing upward and push it onto the clips see Figure 95 The mixer outlet must point upward to ensure thorough mixing of the eluent 4 Locate the 35 cm 14 in length of black PEEK tubing...

Page 97: ...itial filling of the EGC CO3 Mixer with carbonate eluent Figure 96 Plumbing for Filling the EGC CO3 Mixer with Carbonate Eluent EGC DEGAS EGC IN Connect to Gas Separator Assembly TO DEGAS ELUENT OUT T...

Page 98: ...sary adjust the pressure by adding or removing backpressure tubing 8 Set the EGC 1 concentration to the value required for your application 9 Turn on the EGC 1 current 10 Run at these settings until a...

Page 99: ...Figure 97 ICS 2100 Flow Schematic Carbonate Eluent Generation L L S W P C To the pump Degas In Eluent In Regen In Regen Out Eluent Out Cell In Cell Out Column Heater Suppressor Heat Exchanger Guard D...

Page 100: ...the back of the component panel see Figure 98 3 Connect the mixer outlet line to port P 2 on the injection valve NOTE Backpressure tubing may be required between the injection valve and the EGC CO3 M...

Page 101: ...tem set up for anion analyses and 2 S for a system set up for cation analyses Equilibration time varies and it can take some time to reach these expected values 19 1 EluGen Cartridge Backpressure Requ...

Page 102: ...be less than 0 13 MPa 20 psi You can acquire the pressure readings as a separate signal and plot the readings on the IC system panel 1 Verify that the pump pressure signal is enabled in the Server Co...

Page 103: ...channel plot on the Chromeleon panel a Right click anywhere on the plot section of the panel b Click Signals c Under Available Signals select Channel_Pressure and click Add to move the signal to the D...

Page 104: ...ed range For instructions on how to set up the IC system for stand alone operation with an AS autosampler refer to the TTL appendix in the system operator s manual 22 Pressurizing the Eluent Reservoir...

Page 105: ...d to between 0 55 and 0 83 MPa 80 and 120 psi 2 Push the other end of the tubing onto the inlet of the air regulator 3 Remove the eluent reservoir cap 4 Connect the gas line from the eluent reservoir...

Page 106: ...to the reservoir when the gas source is turned on 3 Turn on the gas source 4 Pull out the air regulator knob and turn it clockwise to pressurize the eluent reservoir 5 Adjust the pressure to between 0...

Page 107: ...wash plumbing after all connections are complete 1 Fill a flush bottle with either deionized water or a combination of deionized water and 10 or 20 isopropyl alcohol Isopropyl alcohol will inhibit bac...

Page 108: ...ng is near the bottom of the bottle b Push the 152 cm 60 in piece of clear tubing onto the drain tube on the top of the primary pump head This is the waste line Route this waste line through the lower...

Page 109: ...plete the installation of the kit now refer to For a brief description of the steps required to begin processing samples using Chromeleon refer to Section 24 For details about using Chromeleon refer t...

Page 110: ...is turned on for the first time a Windows message box appears asking for a USB configuration file cmwdmusb inf Possible Cause The USB cable was connected and the power turned on before Chromeleon was...

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