Sample Processing Overview
Doc. 065294-01 3/09
105
e.
Push approximately 5 cm (2 in) of black 0.25-mm (0.01-in) ID tubing
(P/N 057057) into the end of the waste line.
NOTE The black tubing creates a flow restriction.
Assuming a height difference of about 76 cm (30 in)
between the flush bottle and the waste container,
this setup provides a flow of one drop of flush
solution every 5 to 30 seconds.
f.
Place the end of the waste line in a waste container.
g. This completes the piston seal wash installation. Periodically refill the
flush bottle and empty the waste container as required.
This completes the IC system installation procedure. For operating instructions,
refer to your IC system operator’s manual. The manual is on the Dionex
Reference Library CD-ROM (P/N 053891).
If you will be installing the RFIC-ER Startup Kit, complete the installation of the
kit now. refer to
For a brief description of the steps required to begin processing samples using
Chromeleon, refer to
. For details about using Chromeleon, refer to the
Chromeleon Help.
24.
Sample Processing Overview
You can use Chromeleon to create a sequence of sample injections to be processed
automatically.
•
For each injection, the sequence includes a program with commands and
parameters for controlling the IC system and autosampler (if installed) and for
acquiring sample data. To create a program, click
Create Program
on the
Sequence Control
panel on the panel tabset.
•
The sequence also includes a quantification method for peak identification
and area determination. To create a quantification method, select
File > New
and select
Method File
from the list.
•
To create the sequence, click
Create Sequence
on the
Sequence Control
panel on the panel tabset. In the sequence, specify samples to process, the
names of the program and quantification method that you created, and other
