6-6
CELL-DYN
3000 System Operator’s Manual
9140240E — May 1995
Calibration
Chapter 6
Whole Blood Calibration Guidelines
Introduction
Calibration with Fresh Whole Blood Samples is an alternative to
calibration with a Commercial Calibrator. This section explains the
determination of reference values and gives requirements for Whole
Blood Samples and calibration with them.
Sample Requirements for Fresh Whole Blood
The following requirements should be observed for fresh whole blood
samples used for calibration:
1.
The ICSH recommends that fresh samples be less than four hours
old.
1
Sample age must not exceed eight hours at the conclusion of the
calibration procedure.
2.
All parameter values should be within the laboratory’s normal
range. The following ranges are programmed for the reference
values that may be entered in the Auto-Cal program. Results
exceeding these limits cannot be entered.
WBC
2.0
–
25.0
K/
µ
L
RBC
2.00
–
6.50
M/
µ
L
HGB
4.0
–
24.0
g/dL
MCV
70.0
–
100.0
fL
PLT
50.0
–
600.0
K/
µ
L
3.
All cellular morphology must be normal.
4.
No known interfering substances should be present (e.g., lipemia,
icterus, drugs).
5.
All samples must be properly collected in the EDTA anticoagulant
used by the laboratory.
6.
Each tube should contain at least 90% of the nominal collection
volume of blood.
1
Determination of the Reference Values
Reference values for Whole Blood Calibration should be determined
according to the following International Committee for Standardization
in Hematology (ICSH) recommendations.
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