CELL-DYN
3000 System Operator’s Manual
3-31
9140240E — May 1995
Chapter 3
Principles of Operation
Action:
Review the MCV and the PLT histogram. If the MCV is low
and/or the histogram indicates an overlap (poor separation at
the upper discriminator) in the RBC and PLT populations,
review a stained smear to determine the cause and confirm the
PLT count.
LURI (Lower and Upper Region Interference) — displayed next to the
MPV result
Cause:
Interference is present in both the upper and lower regions of
the PLT histogram.
Action:
Follow the guidelines given above for the LRI and URI flags.
PLT — displayed next to PLT result
Cause:
The relationship of the absolute PLT count and the PLT
histogram did not meet the expected criteria.
Action:
Repeat the sample. If the flag remains, verify the PLT count.
Suspect Population Flags
These flags are generated when the instrument’s evaluation of the
measured data for a particular parameter or group of parameters indicates
the possible presence of an abnormal subpopulation. A stained smear
should be reviewed whenever a suspect population flag is present.
Therefore, instructions for interpreting these flags should be incorporated
into the laboratory’s review criteria for abnormal samples.
NOTE: The word SUSPECT will be displayed and printed
above any displayed WBC suspect population flags.
WBC Flags
BANDS — displayed next to the NEU result
Cause:
1.
The count in the region of scatter (on the 0
°
/10
°
plot) where bands
are typically located is >12.5% of the total WBC count.
2.
The ratio of suspected bands to mature neutrophils is >50%.
3.
The CV of the neutrophil cluster on the 0
°
axis exceeds expected
criteria.
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