PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
95
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Set the correct focus value of the desired preview position by clicking
SetI1
,
SetI2
, or
SetI3.
If you
want to go back to any of the previously set focus points, click the appropriate button. To delete
the stored values, click
Clear Values
.
The software calculates a coarse focus level from the three values determined this way as a basis
for the fine focusing routine. You can only define the start parameter here. This will, however,
speed up the focusing process.
If you deselect the
Use fine focus
checkbox, the software immediately starts scanning on the
basis of the interpolated values, without pre-fine-focusing.
To define focus limits, select
Limits of the range
. Set the upper and lower limits by selecting the
desired value in the selection boxes. By using
± Range
, the lower limit value is set to -32 of the
current focus value and the upper limit value is set to +32 of the current focus value. This is useful
if
Auto Focus
does not find the focus on the specimen, but on the coverslip. Also, this makes the
focusing sequence faster.
For manual focusing define the manual focus step size by adjusting the
Step size
slider and use
the two arrow buttons to focus manually. This is useful when you are interested in an area that
remained blurry with
Auto Focus
(for example, the specimen is thicker in that area). This does not
influence the way slides are digitized in Auto focus mode.
Figure 82: Focus indicator panel
5.2.2.3
Exposure indicator
Exposure time for the live image can be calculated by using the
Calculated Exposure
function
. It is available to switch between microsecond, millisecond, and second units for the reading
of exposure time.
Figure 83: Window of Exposure Indicator in Fluorescence scan mode
Select the types of filters you want to use during scanning. You can select the filter types and th e
number of channels to be used. The values in the
EXC
column define the excitation of the
Lumencor illumination, in
TW
define exposure time weight, and in the
DG
the amount of digital
gain is displayed. It is also available for you to enable Extended Focus (
EF
) and/or Z-stack (
ZS
)
functions in this field.
