PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
80
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Important!
It requires lots of time (since it cannot find focus neither on the edges, nor on specks) to
digitize coverslip edges. Select
Remove coverslip
function to eliminate those areas.
Warning!
Do
not
use
Remove coverslip
function on TMA slides! The layout of TMA slides may mislead
the detecting algorithm.
o
Fill holes inside the scanmap
–
If selected, the holes inside the specimen will
also be scanned. Often these holes contain small sample objects that
otherwise would not be scanned.
o
Remove specks
–
If selected, the scanner does not digitize objects smaller
than the predetermined size. This helps filtering specks and other
uninteresting objects out. Set the maximum size of specks not to be digitized
by adjusting the
Speck size
slider.
o
Dilate mask
–
Adjust the slider to enlarge or decrease the scanned area. The
edge is extended discretely by 100 µm each time.
Scanmap option
The previously saved scanmap can be loaded for use. Select one of the following as
Scanmap
source
:
Figure 65: Scanmap source
•
Use Threshold:
Preview settings set in Microscope Controller Panel / Preview
window are applied to create a scanmap.
•
Loaded scanmap:
This function is useful if the slides contain areas of interest that
cannot be detected by the preview camera. This function along with saving
profiles helps to scan all slides with the most adequate settings.
To load a mask, click
first, then browse for the scanmap to be loaded.
