PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
86
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Preview Types
In FL Microscope mode the following additional preview modes are available:
o
Brightfield preview
–
a normal type of illumination for scanning. This
option is recommended when scanning in Brightfield mode.
o
Darkfield preview
–
preview image is created with transmitted Darkfield
illumination. The preview is taken from the center of the sample, and the edge
of the slide might be not visible in some cases. Make sure to select this option
in Fluorescent mode.
o
Pre-scan
–
creates a fast FL preview image that is displayed in the Profile
Wizard window. Scanning process takes a couple of minutes only, and it
cannot be interrupted.
o
Pre-scan high-resolution
–
creates a High-Resolution fast FL preview
image of every FOV within the selected area (in single layer DAPI, with an
illumination time of 20ms) that is displayed in the Profile Wizard window.
Scanning process takes a couple of minutes only and cannot be interrupted.
o
Pre-scan + high-resolution
–
creates a High-Resolution fast FL preview
image of every FOV within the selected area (in single layer DAPI, with an
illumination time of 20ms) that is displayed in the Profile Wizard window.
Scanning process is initiated with a simple Pre-Scan, the located sample with
auto threshold is selected, and finally, an HR Pre-Scan is launched.
Remove Coverslip
If selected, the edges of the coverslip are not detected as specimen to be scanned. Depending on
the threshold level, it is possible that the edges of the coverslip are detected as areas to be
digitized.
Figure 72: Preview image with and without coverslip edge
