3DHISTECH PANNORAMIC MIDI RX 1.0, User Manual

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PANNORAMIC® CONFOCAL RX 1.0 SCANNING MODES AND SETTINGS
MAY 13, 2021 78
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
• Thresholding
The manually set threshold value is applied during scanning. The preview of the
loaded slide is displayed in the Preview window. The set value has effect on the
preview image. If threshold value is modified in the Microscope Controller
preview window, that value is automatically applied in the profile in the Profile
Settings panel.
o
Specimen threshold
o
Manually set value : This sets the sensitivity of the specimen detection. To set
the desired threshold value manually, change the setting in the Specimen
threshold level selection box in the range of 0-255. The default value is 212.
Smaller values result in a more sensitive scanning, meaning that more areas
will be digitized, however, this may also include specks or other imperfections
of the slide. Greater values result in a less sensitive scanning, but it is possible
that some of the smaller specimen areas will not be digitized.
Auto Threshold: By clicking the specimen will automatically be detected in
the preview image.
Caution!
Make sure that the detection result is acceptable for you. Otherwise, set the threshold value
manually or append missing areas with the help of appropriate functions in the
Preview
window.
o
Use marker with threshold
If the specimen cannot be identified as required for digitization, (for example,
weak contrast), it can help to encircle completely the specimen with a black
marker pen. You have to draw a full circle with the pen to enable the
detection. Marker pen detection will make specimen detection easier by
limiting the scanned area. If this option is selected, only the area encircled
with a marker pen will be scanned. You can define the threshold level by
changing the threshold value in the
Use marker with threshold selection box
in the range of 0-255. This sets the sensitivity of the specimen detection inside
the marker area. To set the desired threshold value manually, change the
setting in the
Specimen threshold selection box in the range of 0-255. The
default value is 105.