PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
75
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Figure 59: Filter table
Objective settings
This field displays the objective used for scanning.
Figure 60: Objective settings
•
Objective type
o
20×
or
40×
•
Confocal mode
Select the mode you want to apply from the
Confocal mode
drop-down list:
o
True confocal
–
Normal confocal image is created.
o
Semi confocal
–
Mixed-effect mode, halfway between confocal and widefield
modes. Disk is applied (High/Mid/Low-resolution grid) to create a calibration
error-free cut image with less illumination deriving from layers that are out of
focus (on the other hand, image is brighter than in Confocal mode, and darker
than the image created in transformed widefield mode).
o
Widefield
–
This option is recommended to gain less illumination loss, the
resulting image has no diffraction noise, no plane-parallel shift, nor calibration
error. Scanning time can be four times faster when digitizing faint samples.
•
Confocal disk position
o
Confocal disks have different grid density that affects the confocality (
High-
resolution grid
/
Mid-resolution grid
/
Low-resolution grid
). Higher grid
density results in better confocality, and lower light intensity. The default
setting is
Auto
, and the predefined confocal disk position for the selected
objective type will be used.
