PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
81
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
•
Custom scanmap:
The scanmap drawn on the preview image is used for scanning.
If the
Use selection box restriction
function is activated, only the area inside the
box is scanned. It is useful if you only want to scan the area that is equivalent with
the size of the coverslip, so external specks will not be scanned.
Multilayer mode
•
Single layer mode:
If none of the filters are selected in the Filters table for
multilayer scanning, this mode is active and can be selected, and Multilayer mode
turns inactive.
•
Extended focus:
By choosing the
Extended focus
option, the software selects the
sharpest image from each focus level for each image field and combines them into
one single image. Therefore, those parts that were blurry in auto/manual focus
will also be sharp. This method guarantees the maximum depth of sharpness;
however, it reduces digitization speed.
•
Z-Stack:
For
Extended focus
and
Z-Stack
scanning methods, you can determine
the desired number of focus levels with the
Levels
selection box. The current
focus value is the initial level. If you set
5
for example, then the initial level, plus
four (two focus levels above and below the FOVs) are captured. Therefore, it is
advised to choose an odd number for the focus levels. Separate layers are created
and stored this way and can be opened with Viewer applications. The Z-Stack slide
contains the extended focus layers.
Figure 66: Multilayer mode settings
Step size
determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are:
Levels
: 7;
Step size
: 5.
The number of iterations the Deconvolution algorithm is run can be determined in
the
Iteration number for deconvolution:
value box.
NOTE:
The purpose of deconvolution is to produce a sharper image, but the use of
a deconvolution algorithm can significantly increase the time that takes to create
a digital slide.
