PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
76
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Focus
Focus settings for the scanning. Focus modes can be the following:
•
Coarse:
Preset the coarse focus value. By activating the
Coarse focus
function, the
lower limit value is set to -64, and the upper limit value is set to +64 of the current
focus value. You can accept the current Z-value by clicking
Assign Current
.
Notice:
Before entering coarse focus value, turn live image on and set a suitable focus position.
•
Interpolated:
If you select the
Interpolated
option, the coarse focus level is
calculated from three different focus points of the specimen. Carry out a manual
focusing sequence in the preview image on those three points. Set the correct
focus value of the desired preview position by clicking
Set
in the table of
interpolated focus. If you want to go back to any of the previously set focus
points, click the appropriate
Goto
button.
To delete the stored values, click the other proper focus point or turn off the
function. The software calculates a coarse focus level from the three values
determined this way as a basis for the fine focusing routine. You can only define
the start parameter here. This will, however, speed up the focusing process. If you
deselect the
Use fine focus
checkbox, the software immediately starts scanning
based on the interpolated values, without pre-fine-focusing.
Figure 61: Focus settings
•
Auto focus:
Focus value is set automatically to match sample characteristics.
•
Limit the range
: You can define focus limits by specifying a range for focusing.
You can set the upper and lower limits by selecting the desired value in the
selection boxes. This is useful if focusing was not targeted on the specimen, but
on a spot on the coverslip.
