PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
83
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
Caution!
If the specimen area is barely visible, it is possible that the software will analyze that area as
an empty one.
•
Multiple shots
–
Take multiple shots, specified by the value set in the box.
•
Max exposure
–
A limit value can be set to determine that the exposure time
cannot be longer than the time of focusing and calculation of exposure. This is
necessary because the exposure time would be too long when taking image of an
empty field of view. Default value is 2000 ms.
Image Correction settings
Figure 69: Image correction settings
•
Use Flatfield correction
–
If this function is active the scanned slide compensates
itself with a special method (light intensity statistical data, not to modify the
average intensity of the slide. Quantitative measurements can be made on the
slide. The following three options can be selected from the drop-down list:
o
Intensity statistics
–
legacy compensation algorithm
o
Reduce intensity
–
pixel-level analyzation of the acquired image during which
the background of the image is reduced based on the comparison of the
image recorded by the camera and the compensation image.
o
Intensity equalization
–
pixel-level analyzation of the acquired image, a one-
by-one type image compensation method is applied.
•
Extended focus
–
Select the type of extended focus algorithm used during
scanning:
o
Legacy
–
uses original extended focus algorithm, it is recommended for wide-
field fluorescence scanning. Note, that this algorithm is optimized for scanning
large areas, so if the size of the area to be scanned is insufficient, the
correction might not be perfect.
o
Maxima
–
the final image is made up from all of the layers created, areas (pixel
groups) with the highest intensity are combined together.
o
AVG
–
an average of the layers is used for the final image.
o
Max-AVG
–
the final image is made up from all of the layers created, areas
(pixel groups) with higher intensity than the average are combined together.
