PANNORAMIC® CONFOCAL RX 1.0
SCANNING MODES AND SETTINGS
MAY 13, 2021
59
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES .
•
Z-Stack:
For
Extended focus
and
Z-Stack
scanning methods, you can determine
the desired number of focus levels with the
Levels
selection box. The current
focus value is the initial level. If you set
5
for example, then the initial level, plus
four (two focus levels above and below the FOVs) are captured. Therefore, it is
advised to choose an odd number for the focus levels. Separate layers are created
and stored this way and can be opened with Viewer applications. The Z-Stack slide
contains the extended focus layers.
Figure 38: Focus level settings
Step size
determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are:
Levels
: 7;
Step size
: 5.
The number of iterations the Deconvolution algorithm is run can be determined in
the
Iteration number for deconvolution:
value box.
NOTE:
The purpose of deconvolution is to produce a sharper image, but the use of
a deconvolution algorithm can significantly increase the time that takes to create
a digital slide.
The capacity of deconvolution is minimum 96GB RAM.
Scan settings
•
Stop and go
: A scanning method during which the slide tray stands still during
recording the FOV, then it moves on so that the scanner can record the next FOV.
•
Use Color matrix:
Select an applicable preset color profile to be used for
Brightfield scanning from the drop-down menu. Settings according to the profile
has effect on the color set, color temperature, and gamma value of the scanned
image.
Separate color matrix can be defined and used for each profile and scanning.
Preset profiles
are the following:
•
Calibrated Linear
: Color scheme is defined by calibration (Gamma value is not
included)
•
sRGB standard
: Color scheme is defined by calibration (a standard Gamma value
of 2.2 is applied)
•
sRGB standard Microscope Like
: Color scheme is defined by calibration (standard
Gamma value / White Point: D50)
•
Legacy
: A Color scheme calibrated for the previous software version is applied
•
Custom:
Color profile set in Service is applicable if needed.
