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Lightsheet Z.1
Carl Zeiss
02/2013
000000-1790-528
17
Step 4 is the final step in which the alignment for
all filters and detection module channels is
performed. A progress bar is present at the bottom
of the window. When the procedure is finished the
Finish
button becomes available, press it to save
all adjustments and leave the wizard. This text is
displayed:
“Detector Alignment for all available
filters in progress, please wait.”
If you still encounter pixel shifts between the two
channels after the Automatic Camera Alignment
tool, you can fine tune settings using the Manual
Detector Alignment tool, see section
the Grating Focus for the Automatic or
Manual Detector Alignment Tool
3.2.2
Adjustment – Manual Detector
Alignment
This adjustment is necessary for one or two
detection modules when you recognize a pixel shift
between channels, or when the zoom is not
centered.
The
Automatic Detector Alignment
should always be performed first since it
might already solve the problems.
Furthermore it defines the settings for the
combination Channel 1 (Cam1) and
position 1 of the Emission Selection Filter,
which should be used as the reference
during the Manual Detector Alignment.
The
Zoom
in the
Acquisition tool window
is fixed to 1 while performing the Manual
Detector Alignment.
Fig. 9
Automatic Detector Alignment,
third step
Fig. 10
Manual Detector Alignment
window
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