![background image](http://html1.mh-extra.com/html/zeiss/lightsheet-z-1/lightsheet-z-1_operating-manual_927692303.webp)
Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
133
3.3.15.3
Structured Illumination –
Experimental PSF
This function allows determining the popint-spread
function (PSF) of sub-resolution beads and using
this experimentally determined PSF as input for
Structured Illumination processing.
Select the
Experimental PSF
tab to select the
Experimental PSF
Processing tool (Fig. 212).
Activate the image (a 3D stack of sub resolution
beads) you want to process in the image container
and press the
Select
button for
Input Image
to
load the image. Then expand the
Method
Parameters
tool window to have access to setting
parameters (Fig. 213).
You can load a 3D stack of a wide field
image or a non-processed raw data SIM
image. LSM image formats are not accepted
to determine the
Experimental PSF
.
Pressing the
Default
button will save the present
parameter values as default. By pressing
Reuse
,
the currently saved parameter values will be
loaded.
When the
Symmetrical
check box is ticked, a
radial symmetrization of the PSF will be performed.
That means that a perfectly round PSF is
calculated, even out of an oval PSF.
When the
Damp edge
check box is ticked, the
edge of the PSF will be smoothed (with a smooth
transition to zero at the edge).
The
PSF sampling
pull down menu allows you to
interpolate the result to different degrees. Since
the resulting PSF is an average of many PSFs
corresponding to the localized beads and every
bead is localized with the sub-pixel accuracy, one
can obtain after averaging a PSF with higher
resolution. You can average the PSF by
1
(pixel size
of the resulting PSF is the same or
1
times as in the
original image),
2
(pixel size of the resulting PSF is
2
times smaller as in the original image),
3
(pixel
size of the resulting PSF is
3
times smaller as in the
original image) or
4
(pixel size of the resulting PSF
is
4
times smaller as in the original image) times
(Fig. 214).
There are different filters that can be set by
corresponding sliders or spin input boxes to select
sub-resolution beads.
Fig. 212
Structured Illumination – Experimental
PSF tool selected
Fig. 213
Structured Illumination – Experimental
PSF tool
Fig. 214
PSF sampling drop down menu
Содержание Lightsheet Z.1
Страница 1: ...Lightsheet Z 1 Operating Manual February 2013 ZEN 2012 black edition ...
Страница 4: ......
Страница 170: ......
Страница 427: ...Lightsheet Z 1 Overview ...