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CHAPTER 2 - SAMPLE PREPARATION
Carl Zeiss
Tips, Troubleshooting and Additional Information
Lightsheet Z.1
40
000000-1790-528
02/2013
4.3
Suggested Additional Sources of Information
Chemicals
•
Agarose:
−
Molecular biology grade, for routine use, SIGMA, Ref. A-9539
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Type VII, low gelling temperature, SIGMA, Ref. A-4018-50G
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"Agarose Low Melt" (no 6351.1) from Carl Roth http://www.carlroth.com (from the US please
contact Brunschwig Chemie B.V., Amsterdam, NL (e-Mail: [email protected])
•
Gelrite Gellan Gum, SIGMA, Ref. G-1910
•
Glycerine anhydrous, AppliChem, Ref. A3552,1000
•
Nail Polish, any cosmetic shop near you
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PBS, local supplier
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Distilled water, local supplier
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Ethanol, local supplier
•
Companies:
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Sigma- Aldrich (http://www.sigmaaldrich.com/)
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Applichem (http://www.applichem.de/)
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MP BIomedicals (http://www.mpbio.com/)
−
Merck KGa (http://www.merck.de/)
Materials
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Capillaries
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100 µl, color code Blue, Brand GmbH, Ref. 7087 45
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200 µl, color code Red, Brand GmbH, Ref. 7087 57
•
Companies:
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Brand GmbH (http://www.brand.de/en/home/)
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SpectraGlass (http://www.spectraglass.com/)
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Harvard Apparatus (http://www.harvardapparatus.com/)
•
Syringes
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1 ml, BD Plastipak, BD Biosciences, Ref.300013
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0.5 ml, BD Microfine Insulin U100 Syringe, 29 g, Ref. PLA257L
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0.3 ml, BD Microfine Insulin U100 Syringe, 30 g,Ref. PLA470U
−
Braun Omnifix F Solo 1 ml Syringe (PZN 0569881, Ref. 61706)
−
Terumo 1 ml Syringe (Ref. BS-01T)
Содержание Lightsheet Z.1
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