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Lightsheet Z.1
Right Tool Area, Data Management and Storage
Carl Zeiss
02/2013
000000-1790-528
237
5.2
Comments on Data Management
Using the Lightsheet Z.1 system which is designed to acquire fast Z-Stacks from multiple views over a
period of time will easily produce large datasets. To give a rough idea, here are some typical examples:
Parameters
Experiment 1
Experiment 2
Z-Stack:
300 slices
300 slices
Views:
4
4
Time:
1
300 (overnight experiment)
Channel
1
1
Illumination
dual-side
dual-side
Frame
1600 x 1600 pixel
1600 x 1600 pixel
Size
12 GB
3.6 TB
When using two channels, the data will double.
You may want to consider limiting one or the other of the mentioned parameters to reduce data before
starting the experiment. For example:
Cropping the image frame to the necessary pixels without comprising the image information. Keep in
mind that processing of Multiview data might require sufficient landmarks imaged with the specimen
Binning the pixels, if possible, if high resolution is not necessary.
Keeping Z-Stacks small, but taking into consideration enough fiducials in the different views in case a
landmark alignment processing is planned.
To achieve the specified performance of the system, the
Streaming
option in the
Streaming tool
window of the
Maintain tab
has to be active.
Please ensure the following for the drive you will be streaming your data onto:
−
If you have no storage and analysis PC, use the D drive from your PC for system control. Using the
C drive will lead to an unstable system.
−
If you have a storage and analysis PC:
o
Ensure that you are using the optical fiber cable to connect the PC for system control to
the storage and analysis PC.
o
Ensure that the drive of the storage and analysis PC is mapped to the PC for system
control. This will happen automatically when the storage and analysis PC is booted before
the PC for system control is turned on.
o
While streaming data (experiments) onto the storage and analysis PC, do not try to
remove or load any other data from or to the storage and analysis PC. It will slow down
the data stream from the PC for system control and will influence the acquisition speed of
the experiment.
Содержание Lightsheet Z.1
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