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Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
120
000000-1790-528
02/2013
If all parameters and filters are set press the
Apply
button. This will create the PALM image in the image
container and the
PALM
view tab becomes available that allows further processing and analysis of the
image. The container will display PALM image (
HR
channel) as well as the sum wide field image (
SWF
channel) (Fig. 177).
Note that in contrast to the TIRF image, which is the raw image of the point spread functions
(PSFs), the sum wide-field image is the background corrected image of the PSFs and the PALM
image is a vector map of centroids.
Fig. 177
PALM image (left) and sum widefield image (right) after PALM
Processing
Содержание Lightsheet Z.1
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