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Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
150
000000-1790-528
02/2013
You can select the following
Pre-Registration Method
s from the drop down menu:
None
: no Pre-Registration is performed. Registration is then only done with the settings chosen in the
Registration Method menu.
Translation
: The Views are only moved along x, y, and z axis.
Trans Rotation X
:The Views are moved along the x, y, and z axis as well as rotated around the x axis of
the image.
Trans Rotation Y
: The Views are moved along the x, y, and z axis as well as rotated around the y axis of
the image.
Trans Rotation XY
: The Views are moved along the x, y, and z axis as well as rotated around the x and y
axis of the image.
Hardware Rotation
: The Views are rotated solely based on the information from the rotation
positioning of the sample holder which are saved in the metadata.
Trans Hardware Rotation
: The Views are rotated solely based on the information from the rotation
positioning of the sample holder which are saved in the metadata. Additionally the x, y, and z axis are
moved to match the Views.
Correlation Hardware Rotation
: The Views are rotated solely based on the information from the
rotation positioning of the sample holder which are saved in the metadata. Additionally the x, y, and z
axis are moved to match the Views. Cross-correlation is used to determine the translation in x, y, and z.
To select the
Registration Method
, use the drop-down menu.
Use Pre Register Only
: No Registration process is performed; only the result of the Pre-Registration is
used.
Translation
: The registration will correct along the x, y, and z axis only.
Trans Rotation X
: The registration will correct along the x, y, and z axis and also the rotation along x.
Trans Rotation Y
: The registration will correct along the x, y, and z axis and also the rotation along y.
Trans Rotation
: The registration will correct along the x, y, and z axis and also the rotation along x
and y.
The
Interpolation
function is the technique by which the pixels from the different views are relocated
into the new registered image. With the drop-down menu, you can choose between
Nearest
Neighbour
,
Linear
, or
Cubic
algorithm for this task.
If your data was acquired with dual side illumination which has not yet been fused, the method to fuse
both illumination sides is available in the
Dual Side Fusion
drop-down menu (
Mean Fusion
,
DFT
Fusion
).
Mean Fusion
: The average intensity for each pixel is calculated using the values from both illumination
sides.
DFT Fusion
(Discrete Fourier Transform Fusion): The Fusion is performed based upon the published
method ("Multiple imaging axis microscopy improves resolution for thick-sample applications", Jim
Swoger, Jan Huisken, and Ernst H. K. Stelzer, OPTICS LETTERS / Vol. 28, No. 18 / September 15, 2003).
Fine structures and details are more visible after fusion, which might be worsened when using Mean
Fusion. However, this process can create noticeable artifacts in the resulting image.
Содержание Lightsheet Z.1
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