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Lightsheet Z.1
Center Screen Area / Image Containers - Display and Image Analysis
Carl Zeiss
02/2013
000000-1790-528
229
4.10.4
Overlap Coefficient after Manders
(Manders, Verbeek and Aten, J. Microscopy 169:375-382, 1993)
∑
∑
∑
=
i
i
i
i
i
i
i
Ch
Ch
Ch
Ch
r
2
2
)
2
(
*
)
1
(
2
*
1
−
Another parameter used to quantify colocalization in image pairs
−
Insensitive to differences in signal intensities between the two channels, photo-bleaching, or
amplifier settings
Value range 0 – 1 (0: no colocalization, 1:all pixels colocalize)
4.11
Profile View
This function allows to
−
display the intensity distribution of an image
along a straight or curved line,
−
show the intensity values in table form and
copy table to clipboard or save as text file,
−
show separate profiles for each channel in a
multi channel image.
The settings of the
Dimensions
,
Display
,
Player
and
Graphics
view options control blocks apply.
The additional view-specific
Profile View Option
control block is shown in Fig. 341.
Any changes done with this View Option control block are effective immediately.
The content of the overlay plane is temporarily hidden while the toolbar is displayed.
The
Profile
View can also be used online during imaging.
•
Draw a Profile line in the image display to define a profile. The intensity curves are shown in a separate
graph (see Fig. 342).
•
In the
Profile
View Option control block you can select the width and type of the profile line. The
color setting is taken from the
Graphics
View Option control block.
•
You can place two markers on the profile line to measure differences in intensities and distances in the
XY plane.
•
Tick the
Profile in Image Display
block to see an intensity graph superimposed on the image
(see Fig. 343).
Fig. 341
View Option control block - Profile
View
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