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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
45
The settings for multidimensional acquisition are accomplished within the tool group
Multidimensional
Acquisition
for all functions. If available, the settings are displayed beside the function, e.g. how many
images are taken for the time series (Fig. 53). Corresponding tool bars within the tool groups only
become available if the box of a multidimensional function is checked; otherwise the tool bar is not
displayed (Fig. 54). The
graphical display
field above the
Start Experiment
button shows a
representation of the experiment type with its dimensions shown. It also lists the estimated size of the
final multidimensional image.
Note that the Multiview function needs the Z-Stack function for a useful experiment and will
activate the Z-Stack function as well. The latter is activated and greyed out.
Pressing the
Start Experiment
button starts the image acquisition, taking into account all activated
functions. The progress of the experiment in regards to which image the system currently acquires will be
shown updated to the right of the functions if available (Fig. 55).
When
Streaming
is activated in the
Streaming tool
window under Maintain (see section
), pressing Start Experiment will open a Windows explorer window. Here
the file name and location can be defined as to where the experiment will be saved during the
acquisition.
Upon pressing the
Start Experiment
button, it changes into a
Finish current step
button, whereas the
graphical display
field changes into a
Stop
button. Pressing the
Stop
button immediately finishes the
acquisition. Pressing the
Finish current step
button will interrupt the multidimensional acquisition after
the currently performed step; e. g. when performing a Z-Stack over time it will finish the current Z-Stack
and then stop the experiment.
Fig. 54
Multidimensional Acquisition Tool group – all possible tool tabs
displayed
Fig. 55
Multidimensional input field – display of the current status 208 from
328 slices taken from the 2nd of 10 images of the time series and the
5th view of 6
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