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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
81
Step 4: Select number of views and z-step interval and click "finish"
In this interface you define the number of views by entering the number of
Rotations
within the input
box or using its arrows (Fig. 123). This will lead to the following angles:
−
Rotations: 2
0° angle and 90° angle
−
Rotations: 3
0° angle, 90° angle and 180° angle
−
Rotations: > 3
a full 360° rotation is evenly divided by the number of rotations.
You can set the
Interval
for the Z-Stacks either by typing a value into the box or using its arrow buttons,
or use the
Optimal Interval
button (see as well Z-Stack tool tab section
). Afterwards, press the
Finish
button to complete the
Quick Setup
; the window
will close and the resulting Views are displayed in the
Multiview Setup
tool tab View list.
Be aware that the
Zoom
setting will change when the volume, defined in step 2 and 3 of the
Multiview Quick Setup, requires a larger field of view then with the
Zoom
set in the
Acquisition
tool window. The Multiview Quick Setup uses the higher value of either the largest possible
diagonal through the volume or the length of the y-axis as a reference to determine the needed
field of view, hence the Zoom.
When the
Zoom
setting is changed during the Multiview Quick Setup, the alignment of the light
sheet must be checked and adapted. Following the rule, the light sheet adjustment is the last step
before image acquisition. Please see as well section
3.2.8 Acquisition Parameter – Channel
for
light sheet adjustment and the
Light Sheet Auto-Adjust
routine.
3.2.13
Multidimensional Acquisition –
Information on Experiment
If the
Information on Experiment
tool tab is
expanded, the Information on experiment panel
becomes available (Fig. 124). This tool displays the
information on the current experiment in terms of
−
Channels / Bit Depth
−
Dimension
−
Required Disk Space
−
Duration for 1 frame
Any changes in the settings will be automatically updated in regard to the displayed parameters.
Fig. 123
Multiview Quick Setup, Step 4
Fig. 124
Information on Experiment tool tab
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