Zeiss Lightsheet Z.1, Руководство по эксплуатации

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CHAPTER 2 - SAMPLE PREPARATION
Carl Zeiss Sample Mounting for LSFM Lightsheet Z.1
20 000000-1790-528 02/2013
2.3.2 Molding and Mounting Supports
As described previously, there are several options to prepare a sample and therefore several options to
manipulate and mount it. Initially, readily available products found in cell biology laboratories: syringes,
capillaries or pipettes were used to mount samples. These components are all commercially available,
cheap and convenient for LSFM sample preparation. However, they still need to be prepared for the
specific needs. Plastic syringes exist in various sizes (0.2 ml, 0.3 ml, 0.5 ml, or 1 ml) and have tight
plungers that easily allow pumping and movement of the agarose rod used to embed the sample.
They can also be used to hang the sample by effectively using the plunger and syringe body as forceps.
The sample holder disc for syringes (Fig. 14/
I and K ) provided should be used in this case. Moreover, they
can be purchased sterile for single use applications.
In the case of Lightsheet Z.1, the sample kit is provided with four types of color coded capillaries with
matching plungers (Fig. 14/
A and B ) and color coded sleeves to fit perfectly each type of capillary to the
sample holder (Fig. 14/
C
). The typical protocol of sample mounting is a two-step process of choosing
carefully your sample mounting system based on your sample (size, agarose/sample ratio…) then to
assemble it (e.g. tip+capillary) beforehand. Prepare it (e.g. agarose) and insert it in the
Lightsheet Z.1 upper sample opening (Fig. 14/
G
and
H
).