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CHAPTER 2 - SAMPLE PREPARATION
Carl Zeiss
Introduction
Lightsheet Z.1
4
000000-1790-528
02/2013
Table 1
Topic
Subtopic
Sample/Model
Organism
Technique/ LSFM
implementation
Reference
Physics
Technical set up of MISERB
Structured illumination
Light Sheet Characteristics
Image formation
Image View fusion
Fluorescent beads
Mouse cochlea
Fluorescent beads
Caenorhabditis elegans
live sea urchin embryo, live
Danio rerio
embryo
MISERB
sTSLIM
SPIM
DSLM
LSFM
Fahrback et al, 2010
Schroter et al, 2011
Ritter et al, 2008
Olarte et al, 2012
Rubio-Guivernau et al,
2012
Biochemistry
Laser Microsurgery
Microtubule dynamic instability
mRNA nuclear export
Heterochromatin dynamics
Imaging of engineered gene
expression
In vitro microtubules
In vitro microtubules
Chironomus tentans
Salivary
Glands
MCDK cells,
Drosophila
melanogaster
Drosophila melanogaster
SPIM
SPIM
SPIM
LSFM (FCS)
SPIM
Engelbrecht et al, 2007
Keller et al, 2008
Siebrasse et al, 2012
Capoulade, 2011
Ejsmont et al, 2009
Microbiology
Marine microbiology
Various bacteria, protozoa etc.
LSFM
Fuchs et al, 2002
Cell biology
Adaptive optics to improve
imaging performance
Intracellular imaging
Nuclear protein localisation
Imaging large living samples
Tumour spheroids
Mammalian cell organelles
Cellular spheroids
MCDK cell cysts
waoSPIM
Bessel beam plane
illumination
SPIM
SPIM
Jorand et al, 2012
Planchon et al, 2011
Zanacchi et al, 2011
Verveer et al, 2007
Plant Biology
Live imaging of root growth
Consecutive imaging of vertically
growing root
Arabidopsis thaliana
Arabidopsis thaliana
DSLM
SPIM
Maizel et al, 2011
Sena et al, 2011
Developmental
Biology
Imaging of developing organs
Embryogenesis visualisation
Zebrafish development
Cell identity lineaging and
neurodevelopmental imaging
Gene Expression: hour glass
model verification
Danio rerio
heart valve
Drosophila embryo
Danio rerio
Caenorhabditis elegans
Drosophila melanogaster
SPIM
SPIM
mSPIM
iSPIM
SPIM
Scherz et al, 2008
Huisken et al, 2004
Kaufmann et al, 2012
Wu et al, 2011
Kalinka et al, 2010
Physiology
Middle ear structure
3D reconstruction of inner ear
Brain in vivo imaging
3D reconstruction for
morphological analysis
Scan of whole brain
Neural network imaging
Sectioning of thick tissues
Imaging neuronal activity
Imaging of immunolabelled
receptors
Optical sectioning
Plastic Phantom Structure
Cavia porcellus
Microspheres
Bast's valve
Mouse brain
Mouse brain
Mouse cochlea/zebrafish inner
ear, brain/ rat brain
Mouse vomeronasal cells
Mouse
Meriones unguiculatus
cochlea, Hippocampus reidi
head,
Xenopus laevis
(HR) OPFOS
OPFOS; LSFM
miniSPIM
OPFOS
LSFM
Ultramicroscope
TSLIM
OCPI
SPIM
OPFOS
Buytaert et al, 2007
Hofman et al, 2009
Engelbrecht et al, 2010
Hofman et al, 2007
Mertz and Kim, 2010
Dodt et al, 2007
Santi et al, 2009
Holekamp et al, 2008
Klohs et al, 2008
Buytaert et al, 2012
Содержание Lightsheet Z.1
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