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Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
128
000000-1790-528
02/2013
If the stack is large, available memory might be
limited. In this case a
Warning
is issued,
recommending the usage of the
3D large
mode
(Fig. 197). In this case, the stack is subdivided into
individual smaller stacks, which are individually
processed. The result images of the individual
stacks are than automatically combined into one
output image.
If one selects a region of interest (
ROI
) in the
image, than that
ROI
will be used for image
processing. This also can help in reducing memory
problems when processing huge stacks.
In the
Processing
drop down menu one can select
between
Automatic
and
Manual
processing
(Fig. 198). If
Automatic
is selected, pre-set filter
and parameter values will be used.
If
Manual
is selected these filters and parameters
become accessible to be changed and the
Manual
selection
field becomes accessible (Fig. 199).
The
Automatic
mode is recommended for
images with high modulation contrast. In
case the modulation contrast is low one
might benefit from changing the pre-set
values.
In the
Channel
drop down menu (Fig. 200)
All
or
single channels can be selected from a multi-
channel image that should be processed. The
Channel
drop down menu is not displayed if the
loaded image has only one channel.
Due to the limited number of rotations more
frequencies and hence higher resolution
information can be obtained exactly at the rotation
angles. The directions between the rotational
angles contain lesser frequencies. All available
frequency information will be used if the
Max.Isotrop
checkbox is not ticked. This gives rise
to the flower like appearance of the Fourier
transform, which extends wider at the rotational
angles.
Fig. 197
Warning for memory shortage
Fig. 198
Processing drop down box
Fig. 199
The Manual selection field
Fig. 200
Channel drop down box
Содержание Lightsheet Z.1
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