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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
173
3.4.5.2
Adjustment – Automatic Detector Alignment
This adjustment is necessary for one or two detection modules, when:
−
detection modules have been exchanged
−
reflector turrets have been removed and inserted (please restart ZEN before the alignment task)
−
when you recognize a pixel shift between channels
−
when the zoom is not centered.
No sample should be present. The necessary correction plate and a grating is present for each channel
within the system and needs no additional activation.
The Automatic Camera Alignment will align the image center with the detection module center (zoom
center). For two detection modules, it will additionally align both to have overlaying pixels.
After the detection module exchange, first press the
Detector recognition
button
in the
Adjustment tool
window.
After pressing the
Automatic Detector Alignment
button, a window with a wizard will open that
guides you through the process. Press the
Next
button after each step to continue or the
Previous
button to move one step back. If you press
Cancel
you will abort the process without result.
The first step (Fig. 270) automatically positions the
necessary grating into the light path and adjusts
brightness and contrast. A progress bar is present
in the lower part of the window. You can follow
the process in the displayed live image. You will
find the following text below the live image:
"This will start detector alignment for all available
detectors and filter positions in the system. Auto-
adjustment of brightness and contrast, please
wait."
If the live image of the grating shows the
grid pattern out of focus, cancel the
Automatic Camera Alignment wizard and
then manually adjust the focus of the
grating. The necessary steps are described in
CHAPTER 1 HARDWARE
.
In step 2 the movement of the correction plate is calibrated for both channels. There is no interaction
needed. Press the
Next
button to continue after the task is done. The following text is displayed:
"Calibration of correction plate movement, please wait."
Fig. 270
Automatic Detector Alignment,
first step
Содержание Lightsheet Z.1
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