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Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
62
000000-1790-528
02/2013
The initial window (Fig. 89) of the Light sheet
Auto-Adjust
wizard shows a live image of your sample on
the left hand side. The initial beam path and illumination settings are taken from the Channels Tool and
Light Path Tool, so no additional corrections are necessary. The
Specimen Navigator
Tool is present on
the right hand side. Here, use the y-drive only to find a position below or above your specimen where
only the embedding medium is visible. You can control this step by observing the live image. Only the y-
drive should be used during this step, since the conditions for the light sheet adjustment should be as
close to the imaging conditions as possible. Since the embedding medium is an optical element that will
influence the formation of the light sheet, for its adjustment the position and thickness of the embedding
medium should be the same as during imaging. That is easiest to achieve when only the y-position is
changed. The original position of your specimen will be restored after the wizard is finished.
A short description on what to do during this step is given at the bottom of the window (Please use the
y-drive to only move above or below your sample, until only the embedding medium cylinder is in view.).
When done press the
Next
button. When you press
Cancel
you will leave the wizard without any
changes.
Fig. 89
Light sheet Auto-Adjust first window
Содержание Lightsheet Z.1
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