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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
125
Once the settings are complete press
Apply
to obtain
the trajectories of the single molecules (Fig. 189).
3.3.15
Processing – Structured Illumination
Licensing
Instrument
standard
optional
LSM
ELYRA
Lightsheet Z.1
Tool accepts data recorded in SIM mode on ELYRA. In data sets containing other
types of image data as well ("mixed mode" files), this tool selectively processes the
SIM data. "Experimental PSF" (requires Z-stacks of point-like objects) and "Fourier
Transform" accepts input other than SIM data as well. See full description of tool set
for details.
The
Processing
Structured Illumination
tool works exclusively for data acquired in the
SIM imaging
mode. It subdivides in 5 subcategories (Fig. 190):
−
Structured Illumination
: calculates the high resolution image from the set of structured images
−
TIRF filtered
: Will apply the TIRF sectioning to the SIM image if the TIRF was added as the last
frame.
−
Experimental PSF
: Calculates the PSF from an image obtained with sub-resolution beads.
−
Modulation contrast
: Determines the modulation contrast from an image with beads to serve as
a diagnostic tool
−
Fourier transform
: Calculates the spatial frequencies and displays them in an image. No phase
information will be available.
Fig. 189
Image with trajectories of single
molecules. The selected trajectory is
boxed
Содержание Lightsheet Z.1
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