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Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
68
000000-1790-528
02/2013
3.2.10.2
Performing a Z-Stack Using Center
Mode
Press the
Center
mode button to open the
Center
definition
panel (Fig. 95). This mode allows
setting the Range of the Z-Stack in relation to the
current
Focus Position
which is indicated as
Offset
.
It is useful when several Z-Stacks of the same
range should be acquired at different positions
within the specimen, as the whole range of the
stack can be set to a different position at once
using the
Offset
function.
The values for number of
Slices
and
Interval
are
taken over from any settings done in
First/Last
mode. The
Offset
defines the
Center
position of
the stack in relation to the
Focus Position
.
The
Slices
input box displays the number of slices
of the stack. This number can be edited by typing
or using the arrow keys. Changing the value for
Slices
leaves the
Interval
untouched, but the total
Range
is adjusted.
The
Interval
input box displays the z-distance (interval) between slices in
µm
. This number can be edited
by typing or using the arrow keys. Changing the value for the
Interval
automatically adjusts the total
Range
. The
Slices
number will remain unchanged.
Pressing the Optimal button
sets
Interval
to a value of half the optical slice thickness. Two adjacent
slices have therefore always a 50% overlap. The optimal interval is displayed in the button in
µm
.
Within the
Keep
display field (only available in
Show all
mode) you have the option to switch between
Keep
Interval
and
Keep Slice
. If
Keep Interval
is checked, the number of slices is adjusted to a new
Range
defined pressing
Set First
or
Set Last
. Likewise, when
Keep Slice
is checked, the value for the
Interval
between the slices is adjusted to a new Range defined by pressing
Set First
or
Set Last
.
The
Positio
n (µm)
input box indicates the current position of the focus. The number can be edited by
using the arrow keys. The focus drive will automatically move to the new position.
Pressing the
Center
button will define the current focus position (as defined in the
Position
(µm)
input
box) as the center position of the stack. After pressing
Center
, the whole range will move by the
difference between of the previous center position and the new one. The number in the
Center
input
box will then equal the number in the
Position
(µm)
input box.
The
Offset input
box allows one to define an offset between the actual focus position as displayed in
the
Position
(µm)
input box and the current center position as displayed in the
Center
input box. One
can edit the number by typing or using the arrow keys. When defining a specific
Offset
, the
Center
position will be recalculated as the actual position plus the offset. Accordingly the range of the Z-Stack is
shifted to a different position in Z. Pressing the
C
button of the
Z Navigation
representation moves the
actual
Focus Position
to the
Center
position and acquires an image at that position. A Z-Stack is then
acquired at the new position when starting the experiment. Pressing
Center
moves the
Center
position
back to the actual
Focus Position
setting the
Offset
again to zero.
Fig. 95
Z-Stack Navigation appearance,
performing a Z-Stack using
Center Mode
Содержание Lightsheet Z.1
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