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Carl Zeiss
Left Tool Area and Hardware Control Tools
Lightsheet Z.1
134
000000-1790-528
02/2013
Feature size
sets the mask size that is used to cut out the bead and to create the
Experimental PSF
.
The extensions are displayed in the
Preview
tab of the image container as circles (Fig. 215).
Noise Level
sets the threshold of the intensity of the sub-resolution beads over background according to
I – M > SD x NL (I = intensity of bead; M = mean intensity of image frame; SD = standard deviation of
image frame; NL = noise level). Lowest number means that everything (even noise) is taken into account;
highest number means only the brightest beads will be used.
Beads quality
evaluates two characteristics, the intensity (first moment) and the size (second moment).
These two moments are calculated for every localized bead. Then the beads are sorted according to these
characteristics, resulting in two sorted lists of beads. The beads that are closer to the medians in both
orderings are assumed to have higher quality. The
Beads quality
defines how far the beads
characteristics (intensity and size) may be away from the median value. The smaller the threshold, the
closer beads should be to the median. Beads quality equals to 0 is likely to result in a single bead right in
the middle. In this way also the bead shape (aspect ratio) is assessed. Lower input number means that
only perfectly shaped round beads will be taken into account. Highest input numbers will take into
account all beads, even ellipsoid shaped ones.
Focal Plane
determines which plane is the focal plane. The default is
-1
(automatic). Here the system
determines the focal plane automatically. The user can also select a frame, which is presumably the focal
plane, by input the number of the frame within the stack.
Fig. 215
Selection of beads in Preview tab
Содержание Lightsheet Z.1
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