![background image](http://html1.mh-extra.com/html/zeiss/lightsheet-z-1/lightsheet-z-1_operating-manual_927692022.webp)
Carl Zeiss
Lightsheet Z.1
18
000000-1790-528
02/2013
No sample should be present when performing this task. When pressing the
Manual Detector
Alignment
button, a window will open (Fig. 10). A grating is automatically inserted into the light path
and a live image of it is shown in a new image container within the ZEN software. The
Set Exposure
button is activated automatically to adjust brightness. All components of the light path can be controlled
in the
Manual Detector Alignment
window. The light source is the LED white light source to ensure
the visibility of the grating independent of the filters in the light path. All other controls for adjusting
imaging settings should be controlled in the tool windows of the main software.
The activated (blue)
Automatic
button loads the
settings which have been previously defined with
the Automatic Detector Alignment for each light
path.
After pressing the
Continuous
button in the
Manual Detector Alignment
window or in the
Main tool tabs
a new image will open with a live
image of the grating (Fig. 11)
When the grating is out of focus, you need
to refocus it for the relevant channel by
performing the steps described in section
3.2.3. To check the focus, zoom into the
image using the
Zoom
function and look at
the center cross within the square (Fig. 12). If
the white portions within the inner most
circle are recognizable, the grating is in
focus.
To zoom into the image you can use these
buttons:
As a reference, the combination of Emission
Selection filter position 1 and Cam 1 should be
taken (see above). This combination should be the
first live image. Here mark a structure close to the
middle of the circled cross using the
Graphics
View control
. This cross will be the reference for
the following steps.
Now change the light path to the combination of
Emission Selection filter and Channel (Cam 1 or
Cam 2) that you wish to adjust. The live image will
now show the grating with the current settings.
Fig. 11
Manual Detector Alignment,
Grating at Zoom 1
Fig. 12
Manual Detector Alignment,
Grating, center part zoomed in
Содержание Lightsheet Z.1
Страница 1: ...Lightsheet Z 1 Operating Manual February 2013 ZEN 2012 black edition ...
Страница 4: ......
Страница 170: ......
Страница 427: ...Lightsheet Z 1 Overview ...