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CHAPTER 2 - SAMPLE PREPARATION
Lightsheet Z.1
Tips, Troubleshooting and Additional Information
Carl Zeiss
02/2013
000000-1790-528
41
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Pipettes
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2 ml serological pipette, FALCON, BD Labware, Ref.35 7507
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1 ml serological pipette, FALCON, BD Labware, Ref.35 7521
Equipments
For the following, please refer to your local lab supply companies
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Heating blocks
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Stereomicroscope
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Scalpels
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Tweezers
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Dissection Needles
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Watch glass
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Sonicator
4.4
References and Further Reading
Buytaert, J.A.N. et al., 2011. The OPFOS Microscopy Family: High-Resolution Optical Sectioning of
Biomedical Specimens. Anatomy research international, 2012.
Capoulade, J. et al., 2011. Quantitative fluorescence imaging of protein diffusion and interaction in living
cells. Nature biotechnology, 29(9), pp.835–839.
Dodt, H.U. et al., 2007. Ultramicroscopy: three-dimensional visualization of neuronal networks in the
whole mouse brain. Nature methods, 4(4), pp.331–336.
Ejsmont, R.K. et al., 2009. A toolkit for high-throughput, cross-species gene engineering in Drosophila.
Nature methods.
Engelbrecht, C.J. et al., 2007. Three-dimensional laser microsurgery in light-sheet based microscopy
(SPIM). Optics Express, 15(10), pp.6420–6430.
Engelbrecht, C.J., Voigt, F. & Helmchen, F., 2010. Miniaturized selective plane illumination microscopy for
high-contrast in vivo fluorescence imaging. Optics letters, 35(9), pp.1413–1415.
Fahrbach, F.O. & Rohrbach, A., 2010. A line scanned light-sheet microscope with phase shaped self-
reconstructing beams. Optics Express, 18(23), pp.24229–24244.
Содержание Lightsheet Z.1
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