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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
119
There will be two filter settings when one performs
grouping before localization:
−
Filters for
Drift Correction
based on fiducial
markers (like gold particles).
−
Filters for
Grouping
the peaks (events).
Expand the
Drift Correction
field to have access
to the
Drift Correction
tool (Fig. 175). If you want
to perform a drift correction the
Drift Correction
check box must be ticked.
Note that for lateral drift correction the system needs to identify at least one fiducial marker based
on the
Drift Correction
filter settings. Fiducial markers could be gold particles deposited on the
glass surface of the dish. If the system cannot find a fiducial, no drift correction will be performed
and in the
PAL-Drift
tab of the image no fiducials will be listed in the
Fiducial
table.
You can set two parameters to be associated with a fiducial by using the sliders or the spin input boxes:
−
Min on time
[
%].
This defines the minimal number of frames out of the total frames acquired in
percentage the molecule has to be on in order to be regarded as a fiducial. E. g. 75 % means that
the molecule has to be on at least ¾ of all the frames.
−
Capture radius [pixel].
This defines the radius around a fiducial in which no other fiducial shoud
be localized in order to be taken as a fiducial. E. g. if 2 pixels was set and within two pixels another
fiducial is present, than both fiducials will be discarded.
Note that the settings of the
Drift Correction
will not influence the
Preview
image.
Note that using
Drift Correction
in the Processing tool is irreversible. Therefore, in the created
image all drift related functions in the
PAL-Drift
tab will be inaccessible and greyed out.
Expand the
Grouping
field to have access to the
Grouping
tool (Fig. 176).
You can set two filters to be taken into
consideration when peaks (events) are grouped:
−
Off Gap.
If peaks within the defined
Capture Radius
will be interrupted by more
than the set value of consecutive frames,
they will not be regarded belonging to the
same molecules.
−
Capture radius [pixel].
If peaks in consecutive frames are not localized within the specified radius
they are not regarded as belonging to the same molecule.
Note that the settings of the
Grouping
will not influence the
Preview
image.
Note that using
Grouping
in the processing tool is irreversible. Therefore, in the created
PAL-
Grouping
tab all functions will be greyed out and not accessible.
Fig. 175
Drift correction panel
Fig. 176
Grouping panel
Содержание Lightsheet Z.1
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