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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
147
Multiview Processing
The check-box next to
Multiview Processing
opens the interface to define the settings for the
Registration
Parameters
, the
Input settings
, and
the
Fusion options
for the data (Fig. 233). Each
interface is accessed by pressing the
button
next to it.
The following datasets can be processed with this
task:
−
Multiview datasets, single side illumination
−
Multiview datasets, dual side illumination,
Dual Side without Fusion
−
Multiview datasets, dual side illumination,
Dual Side with Fusion
−
Multiview datasets, with Deconvolution
−
Multiview datasets, without Deconvolution
The first step is to select the
Registration Options
using the drop-down menu. The choice will
influence the
Parameters
interface.
The
Registration
will generate the transformation
matrices so that all views are matched to overlay.
As a result, a dataset is provided in which all Z-
Stacks of all views should already largely overlay.
The reference at which registrations starts is always
View 1 of the dataset. This image can be used as a
control. If one or more views do not have the same
alignment, registration was not successful and the
parameters must be adjusted.
When
Apply
is pressed and the registration
process has finished, the transformation matrices
are saved as an .xml file (Filename.czi.xml). Only
the transformation matrix is saved if
Apply
is
pressed and
None
is selected under
Fusion
(see
below). This .xml file can be used for the
Use
Registration From File
option.
The
Registration
must always be done before the
Fusion
of the data.
Registration Options
are:
−
Landmark Alignment
−
Intensity Based Alignment
−
Use Registration From File
Fig. 233
Processing – Lightsheet Processing.
Multiview Processing
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