![background image](http://html1.mh-extra.com/html/zeiss/lightsheet-z-1/lightsheet-z-1_operating-manual_927692225.webp)
Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
55
Information about the thickness of the light sheet
with the selected zoom is available when pressing
the
Advanced..
button. A graphical display
(Fig. 73) informs you about the thickness in the
center and the border of the created light sheet in
µ
m within the field of view. To manually change
the thickness of the
Center Thickness
use either
the slider, enter a value into the box, or use the
arrows.
Be aware, this will change the ratio of the light
sheet thickness center to border of the image
frame. If you decrease the
Center Thickness
, the
ratio center to border will be 1: >2; so the light
sheet will be thinner in the center but wider at the
border of the image field (Fig. 75). If you increase
the
Center Thickness
, the ratio will change to
<1:2 accordingly, so the light sheet will have a
more homogeneous thickness in the image field
(Fig. 74).
To return to the ideal light sheet thickness
ratio, use the
Zoom
slider in the
Acquisition Mode
tool window.
Be aware that the Zoom and the Center Thickness
settings are influencing the dimensions of the light
sheet overall. The ratio or the thickness in the
center of the light sheet and at its border should
be ideally 1:2 for the used frame.
The
Select by Region
button opens an additional
window displaying a live image of the sample.
A square region is defined within the frame with
red (y-axis) and green (x- axis) markings. The region
can be enlarged along these axis by dragging the
according side with the mouse. But the square
cannot be moved to an offset within the image.
Within the chosen sub-frame, the optimal light
sheet thickness ratio of the center to the border of
1:2 is created. The thickness of the light sheet at
the center of the chosen square is displayed next
to the
Lightsheet Center Thickness
slider within
the input box.
Fig. 73
Advanced... light sheet thickness menu
Fig. 74
Increased thickness at the center
Fig. 75
Decreased thickness at the center
Fig. 76
Select Light Sheet Thickness by Region
Содержание Lightsheet Z.1
Страница 1: ...Lightsheet Z 1 Operating Manual February 2013 ZEN 2012 black edition ...
Страница 4: ......
Страница 170: ......
Страница 427: ...Lightsheet Z 1 Overview ...