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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
175
3.4.5.3
Adjustment – Manual Detector
Alignment
This adjustment is necessary for one or two
detection modules when you recognize a pixel shift
between channels or when the zoom is not
centered.
The
Automatic Detector Alignment
should always be performed first since it
might already solve the problems.
Furthermore, it defines the settings for the
combination Channel 1 (Cam1) and position
1 of the Emission Selection Filter (see section
), which should be used as a
reference during the Manual Detector
Alignment.
The
Zoom
in the
Acquisition tool window
is set to 1 while performing the Manual
Detector Alignment.
No sample should be present when performing this
task. When pressing the
Manual Detector
Alignment
button, a window will open (Fig. 272).
A grating is automatically inserted into the light
path and a live image of it is shown in a new
image container within the ZEN software. The
Set
Exposure
button is activated automatically to
adjust brightness. All components of the light path
can be controlled in the
Manual Detector
Alignment
window. The light source is the LED
white light source to ensure the visibility of the
grating independent of the filters in the light path.
All other controls for adjusting imaging settings
should be controlled in the tool windows of the
main software.
Fig. 272
Manual Detector Alignment window
Содержание Lightsheet Z.1
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