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Lightsheet Z.1
Left Tool Area and Hardware Control Tools
Carl Zeiss
02/2013
000000-1790-528
135
All sub-resolution beads that will be taken into account will be circled by a white line. Those that do
overlap with their circles or are considered unsuitable (beads beyond the quality thresholds) are outlined
in red and will not be taken into account (Fig. 215).
If
Apply
is pressed an average PSF from all the beads taken into account will be computed (Fig. 216). For
each channel a separate PSF will be displayed. The
Experimental PSF
can be saved (format .czi) and
used for the
Structured Illumination
- Structured Illumination
Processing tool in place of the
Theoretical PSF
. The size and pixel size of the
Experimental PSF
are recalculated and the PSF is
interpolated to match the processed image.
Fig. 216
Experimental PSF in Ortho view
Содержание Lightsheet Z.1
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