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Quick Set-up Guide for the Biomedx Configured Olympus CX43

Summary of Contents for Configured Olympus CX43

Page 1: ...Page 1 v3 01 22 Quick Set up Guide for the Biomedx Configured Olympus CX43 ...

Page 2: ...dx STEPS FOR MICROSCOPE ASSEMBLY When you get your microscope you will find that the various parts are packed in different boxes You will find it advantageous to take everything out of the boxes and lay them on a clean work surface so you can then begin the identification process and assembly Depending on your chosen configuration you may have parts and pieces that differ somewhat from what is sho...

Page 3: ... 6 Putting on the View Head and Eyepieces 7 Plugging in the Camera 8 WiFi 8 Power 9 Video Only Setup 10 Operation Notes Modes of the Condenser 11 Specimen Stage X Y Control Phase Adjusters 12 Focus Knobs Tension Adjustment Stage Stopper 13 HD Camera 14 HDTV Setup 17 Condenser Mode Viewing Tips 19 Using Oil Objectives 20 Supply Reorder Workshops Support 23 ...

Page 4: ...Place onto filter space shown to enhance darkfield view in turret DF mode When your scope shipped there was a top cover over the objective tube opening on the very top of the microscope It is not shown on the scope in the above picture but shown below Fitted inside the plastic cover is a black plastic donut This is a darkfield enhancer When the microscope is being used in darkfield mode up to 40x th...

Page 5: ...ssembly This slider moves an internal prism back and forth When the slider is pushed in 100 of the light is directed to the eyepieces When pulled out 20 of the light remains in the eyepieces and 80 is directed to the video camera To see an image on your camera s TV the slider must be pulled OUT If the bar is only pulled half way out you will only see half an image on your monitor ...

Page 6: ...s for parfocal adjustment These are preset before shipping Parfocal means when you have a focus on your microscope as viewed through the right eyepiece ocular your video image will also be focused If your monitor is not in focus with your right eyepiece scope view you can correct the video focus by loosening the lock screw adjusting the focus screw which will focus the video on the monitor and the...

Page 7: ... the tiny screw holes on the ocular barrel to hold them in place The rubber eyecups can be unfolded for normal viewing as shown here on the left eyepiece or folded down as shown on the right eyepiece when wearing glasses The eyepiece tube assembly can be pulled apart as well as tilted to adjust for your own eyes The left eyepiece tube has a diopter adjustment In use you would focus the microscope ...

Page 8: ... a computer HDMI slot Example of a HDTV input The best imaging will be on a HDTV with 1920 x 1080 resolution To use a computer laptop for image save capture record Plug the WIFI chip into the camera s WiFi USB socket Turn on the camera and go to your computer s WIFI settings to view available networks Find BIOMEDXCAM and connect using password 12345678 The camera will now be able to be found via t...

Page 9: ...mera It s always a good idea to plug your system into a surge protected AC power strip When you are not using your system you can simply switch off the power strip In addition when not using your microscope you can put a cover on it to keep the dust off When using a plastic cover just pop it over the whole scope leaving some room for air to get in the bottom so you don t lock in any moisture Assem...

Page 10: ...ire to temporarily travel light without that assemby you can obtain a short 14 16 gauge wire as shown and you can swap the binocular head with the cameral port cover cap through this procedure Packaged with your trinocular port is a short wire Place this wire into the binocular head receptacle slot as shown here Move the camera port cover cap from the back to the front and lightly tighten the set ...

Page 11: ...a dry blood view to give you less contrast and depth of field This is when using the 2X plan objective so you can see a wider field of view when looking into the eyepieces This is for reflected light fluorescence viewing Scope as shipped is not equipped for fluorescence The most popular Biomedx configuration is set up for non oil microscopy using all condenser modes and will have 2x 4x 10x 20x 40x...

Page 12: ...ated them you would move the pre adjusted phase annulus of the condenser and push it out of adjustment DO NOT do that unless you know what you are doing and have a phase centering telescope and can put the annulus back where it belongs See the manual and review videos on line for reference The phase contrast adjustments were pre set for the phase objectives on your microscope prior to shipping and...

Page 13: ...hting situation Focus knob and stage tension The inner ring on the right focus knob is a stage tension adjustment With your fingertips you can rotate it counter clockwise to loosen the stage tension and focus knob If too loose the stage can vertically drift down by itself and that can be corrected by tightening the tension ring Setting a stop point for the stage This is the pre focus stage stop ad...

Page 14: ...t menus Below is the monitor left edge menu Mouse clicks on Snap or Record will take a picture or begin recording a video to the SD memory card For everyday scope viewing in all modes the Auto Exposure AE mode should be selected and checked With AE set the camera will handle the exposure details for varying light levels The values shown on the panel for the blue highlighted sliders are those you c...

Page 15: ...tly Whether it needs to be tweeked may depend on your monitor Moving the red or blue value a single point up or down with the mouse can be difficult The mouse scroll wheel moves the values at 3 point increments To arrive at the value you want scrolling up from 0 or down from 200 will often land you on the value you want Sharpness setting from 20 150 can all look very good and sharpen the image to ...

Page 16: ... slide so you could calibrate your on screen images to obtain accurate micro measurements Moving the mouse arrow to the bottom edge of the video screen brings up this menu Freezes frame on screen Puts grid lines on screen WDR Wide Dynamic Range not used Compares images Access SD memory card to see image and video files you have captured or recorded Note that while the camera can record the video y...

Page 17: ... resolu on to be be er than all the others on that par cular shelf That was surprising as it was also only 170 More recently I saw a Samsung 32 1080P N5300 series to be the best on the shelf for 250 Basically you don t know how any TV will perform un l using it but in general s ck to the one with the best screen angles and it should work okay Example TV se ngs Auto Brightness Control Off Backlight ...

Page 18: ...some mes it is nice to add a bit of height to the TV when it is on a desktop This can be done with a monitor arm What we use on many of the lab sta ons in the Biotorium classroom is a VIVO stand It is shown here at Amazon for screens up to 27 Vivo also has the same for larger monitors It also comes as a free standing unit ...

Page 19: ... does above and if it does you are all set DARKFILED DF condenser mode using a 20x or 40x objective 2nd image above While darkfield is older technology from phase contrast some individuals studied with others that have used this as a primary method often using dedicated oil condensers and oil objectives With a non oil setup as we have here some adjustment is in order to get to a better darkfield v...

Page 20: ...x oil phase objective will be rotated into place After dropping on the oil you will rotate your oil objective into place and move it back and forth in the oil a few times a millimeter either way to well seat the oil around the objective lens Your turret condenser should be moved to DF mode if not already there Oil Using 50x oil darkfield objective USING OIL OBJECTIVES Having to use oil objectives ...

Page 21: ...ever with the other An iris will give a little more variability to the light as opposed to the fixed aperture of the donut Note that the 50x oil objective has an iris built into it which rotates in either direction When your 50x oil objective is in place over your sample and your condenser is properly set to DF mode with the light intensity turned UP you will need to adjust this objective iris to ...

Page 22: ...PH3 mode Oil Oiling the Condenser The microscope s universal turret condenser has a numerical aperture of 1 25NA so it can be oiled Numerical aperture provides resolving power when magnifying a specimen With objectives up to 9 NA you do not need oil on the condenser When using objectives that go beyond 9NA oiling the condenser might bring a bit more resolution Many people that use oil on the top o...

Page 23: ...1 22 Get supplies and re supply at https store biomedx com Get the latest training info at https biomedx com training Get support current manual and videos at https biomedx com support Support Phone 1 206 577 0037 ...

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