Thermo Finnigan SpectraSYSTEM UV6000LP, Справочное руководство

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3-4 Thermo Electron January 2004
Figure 3.2 UV6000LP Parameters
This method sets the UV6000LP detector for a 5.00 minute run time.
The peaks will likely elute in less than 5 minutes when using a 10-cm,
Hypersil, C8 column. The run time parameter must be adjusted if you
will be using a different column.
The rise time is set to 2 seconds to minimize short-term noise.
Spectral scans will be collected over the wavelength range from 220
to 500 nm in 1nm intervals at a scan rate of 1 Hz. Scans are collected
from 220 to 500 nm to collect the complete spectrum of tartrazine,
which is the compound that gives the LC Test Mix its yellow color.
Tartrazine has a wavelength maximum at approximately 430 nm. The
bandwidth setting for the scan data is 1 nm for optimal spectral
resolution.
The discrete channels are set to monitor at 254, 430, and 220 nm with
a bandwidth setting of 7 nm and a sample rate of 10 Hz. To increase
spectral resolution, the bandwidth setting for the scan data should be
set low. To decrease short-term noise, the bandwidth setting for the
discrete channels can be set higher. Because the scan and discrete
channel bandwidth filters are independent, the bandwidth of the
discrete channels can be set appropriately to decrease baseline noise