January 2004
Thermo Electron
xi
handling, and disposal of both solvents and samples. Become
familiar with the toxicity data and potential hazards associated with
all chemicals by referring to the manufacturers’ Material Safety Data
Sheets (MSDS).
Sample Preparation
Always consider the solubility of your sample in the solvent/mobile
phase. Sample precipitation can plug the column, tubing and/or flow
cell causing flow restriction. This obstruction may result in
irreparable damage to the system. Particulate matter can be avoided
by filtering the samples through 0.45 or 0.2 micron (or less) filters.
Solvent Requirements
Many chemical manufacturers provide a line of high-purity or
spectro-quality reagents that are free of chemical impurities. Routine
filtration of all solvents or eluents through a 0.45 or 0.2 micron (or
less) fluorocarbon filter before placing them in the solvent reservoir
will significantly prolong the life and effectiveness of the inlet filters,
check valves and seals, injector, and column. Typically, HPLC-grade
solvents do not require filtration.
Choose a mobile phase that’s compatible with the sample and column
you’ve selected for your separation. Remember that some solvents are
corrosive to stainless steel. Inert, biocompatible versions of
instruments are also available from Thermo Electron.
Degas the Eluents
Degas your eluent solvents using either the vacuum degassing or the
helium sparging technique. A complete description of these
techniques is found in separate documentation provided with
degassing accessories.
Solvent Disposal
Make sure you have a solvent waste container or other kind of drain
system available at or below the benchtop level. Most solvents have
special disposal requirements and should not be disposed of directly
down a drain. Follow all governmental regulations when disposing of
any chemical.
High-pressure Systems and Leaks
LC systems operate at high pressures. Because liquids aren’t highly
compressible they do not store much energy. Accordingly, there is
little immediate danger from the high pressures in an LC system.
However, if a leak occurs, it should be corrected as soon as possible.
Finally, we recommend that you always wear eye and skin protection
when working on an LC system and that you always shut down the
system and return it to atmospheric pressure before attempting any
maintenance.
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